ArticleAmerican journal of physiology. Lung cellular and molecular physiology2008
AICAR decreases the activity of two distinct amiloride-sensitive Na+-permeable channels in H441 human lung epithelial cell monolayers.
Article in American journal of physiology. Lung cellular and molecular physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Conditioned Medium of Bone Marrow Mesenchymal Stem Cells Involved in Acute Lung Injury by Regulating Epithelial Sodium ChannelsFrontiers in bioengineering and biotechnology · 2021Article
- Lipopolysaccharide Inhibits Alpha Epithelial Sodium Channel Expression via MiR-124-5p in Alveolar Type 2 Epithelial Cells.BioMed research international · 2020Article
- Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro.Pflugers Archiv : European journal of physiology · 2015Article
- Regulation of ion channels and transporters by AMP-activated kinase (AMPK).Channels (Austin, Tex.) · 2014Review
- Mechanism of action of novel lung edema therapeutic AP301 by activation of the epithelial sodium channel.Molecular pharmacology · 2013Article
- Two outward potassium current types are expressed during the neural differentiation of neural stem cells.Neural regeneration research · 2013Article
- AP301, a synthetic peptide mimicking the lectin-like domain of TNF, enhances amiloride-sensitive Na(+) current in primary dog, pig and rat alveolar type II cells.Pulmonary pharmacology & therapeutics · 2013Article
- Downregulation of the renal outer medullary K(+) channel ROMK by the AMP-activated protein kinase.Pflugers Archiv : European journal of physiology · 2013Article
- AMP-activated protein kinase (AMPK)-dependent and -independent pathways regulate hypoxic inhibition of transepithelial Na+ transport across human airway epithelial cells.British journal of pharmacology · 2012Article
- The gasotransmitter hydrogen sulphide decreases Na⁺ transport across pulmonary epithelial cells.British journal of pharmacology · 2012Article
- Gasotransmitters: novel regulators of epithelial na(+) transport?Frontiers in physiology · 2012Article
- Cleavage of endogenous γENaC and elevated abundance of αENaC are associated with increased Na⁺ transport in response to apical fluid volume expansion in human H441 airway epithelial cells.Pflugers Archiv : European journal of physiology · 2011Article
- Amiloride-sensitive sodium channels and pulmonary edema.Pulmonary medicine · 2011Article
- Lipopolysaccharide modifies amiloride-sensitive Na+ transport processes across human airway cells: role of mitogen-activated protein kinases ERK 1/2 and 5.Pflugers Archiv : European journal of physiology · 2010Article
- Endolymphatic sodium homeostasis by extramacular epithelium of the saccule.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Regulation of epithelial sodium channels by cGMP/PKGII.The Journal of physiology · 2009Article
- Regulation of ENaC-mediated sodium transport by glucocorticoids in Reissner's membrane epithelium.American journal of physiology. Cell physiology · 2009Article
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4 authors at 1 institution in 1 country.
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Abstract
Transepithelial transport of Na(+) across the lung epithelium via amiloride-sensitive Na(+) channels (ENaC) regulates fluid volume in the lung lumen. Activators of AMP-activated protein kinase (AMPK), the adenosine monophosphate mimetic AICAR, and the biguanide metformin decreased amiloride-sensitive apical Na(+) conductance (G(Na+)) in human H441 airway epithelial cell monolayers. Cell-attached patch-clamp recordings identified two distinct constitutively active cation channels in the apical membrane that were likely to contribute to G(Na+): a 5-pS highly Na(+) selective ENaC-like channel (HSC) and an 18-pS nonselective cation channel (NSC). Substituting NaCl with NMDG-Cl in the patch pipette solution shifted the reversal potentials of HSC and NSC, respectively, from +23 mV to -38 mV and 0 mV to -35 mV. Amiloride at 1 microM inhibited HSC activity and 56% of short-circuit current (I(sc)), whereas 10 microM amiloride partially reduced NSC activity and inhibited a further 30% of I(sc). Neither conductance was associated with CNG channels as there was no effect of 10 microM pimoside on I(sc), HSC, or NSC activity, and 8-bromo-cGMP (0.3-0.1 mM) did not induce or increase HSC or NSC activity. Pretreatment of H441 monolayers with 2 mM AICAR inhibited HSC/NSC activity by 90%, and this effect was reversed by the AMPK inhibitor Compound C. All three ENaC proteins were identified in the apical membrane of H441 monolayers, but no change in their abundance was detected after treatment with AICAR. In conclusion, activation of AMPK with AICAR in H441 cell monolayers is associated with inhibition of two distinct amiloride-sensitive Na(+)-permeable channels by a mechanism that likely reduces channel open probability.
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