ArticleApplied and environmental microbiology2008
Genetic tools for studying Capnocytophaga canimorsus.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- β-lactamase genes in clinical isolates of Capnocytophaga canimorsus and description of a novel class D β-lactamase, OXA-1422.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Fatal septic shock and Waterhouse-Friderichsen syndrome caused by serovar B Capnocytophaga canimorsus in an immunocompetent patient. Case report and review.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2023Article
- A case of septic arthritis caused byAccess microbiology · 2022Article
- Article
- A small 'lick' will sink a great ship: fulminant septicaemia after dog saliva wound treatment in an asplenic patient.International wound journal · 2017Article
- Identification of Virulent Capnocytophaga canimorsus Isolates by Capsular Typing.Journal of clinical microbiology · 2017Article
- Evidence for a LOS and a capsular polysaccharide in Capnocytophaga canimorsus.Scientific reports · 2016Article
- Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown.Scientific reports · 2016Article
- Article
- Modification of the 1-Phosphate Group during Biosynthesis of Capnocytophaga canimorsus Lipid A.Infection and immunity · 2016Article
- Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth.mBio · 2015Article
- New iron acquisition system in Bacteroidetes.Infection and immunity · 2015Article
- Ingestibility, digestibility, and engineered biological control potential of Flavobacterium hibernum, isolated from larval mosquito habitats.Applied and environmental microbiology · 2014Article
- The N-glycan glycoprotein deglycosylation complex (Gpd) from Capnocytophaga canimorsus deglycosylates human IgG.PLoS pathogens · 2011Article
- A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.Journal of microbiological methods · 2010Article
- Resistance of Capnocytophaga canimorsus to killing by human complement and polymorphonuclear leukocytes.Infection and immunity · 2009Article
- Capnocytophaga canimorsus: a human pathogen feeding at the surface of epithelial cells and phagocytes.PLoS pathogens · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capnocytophaga canimorsus, a commensal bacterium from canine oral flora, has been isolated throughout the world from severe human infections caused by dog bites. Due to the low level of evolutionary relationship to Proteobacteria, genetic methods suitable for the genus Capnocytophaga needed to be established. Here, we show that Tn4351, derived from Bacteroides fragilis, could be introduced by conjugation into C. canimorsus and conferred resistance to erythromycin. By mapping and sequencing a naturally occurring plasmid isolated from a clinical isolate of C. canimorsus, we identified a repA gene that allowed us to construct Escherichia coli-Capnocytophaga shuttle vectors. Most commonly used antibiotic markers were not functional in C. canimorsus, but cefoxitin (cfxA), tetracycline (tetQ), and erythromycin (ermF) resistances could be used as markers for plasmid maintenance in C. canimorsus and even in some other Capnocytophaga spp. Shuttle vectors were introduced into C. canimorsus either by conjugation using the origin of transfer (oriT) of RP4 or by electrotransformation. Taking advantage of the promoter of ermF, an expression vector was constructed. Finally, a method that allows site-directed mutagenesis is described. All these genetic tools pave the way, not only for molecular studies of the pathogenesis of C. canimorsus, but also for studies of other oral Capnocytophaga species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.