ArticleJournal of bacteriology2008
Characterization of spores of Bacillus subtilis that lack most coat layers.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 102 citations in OpenAlex.
- Article
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- Effects of sporulation times in liquid or on plates onJournal of bacteriology · 2025Article
- Bioremediation and Recovery of Lead and Cadmium by Spores of Bacillus subtilis C1.MicrobiologyOpen · 2025Article
- Pathways for accelerated bacterial spore killing with ohmic heating.NPJ science of food · 2025Article
- Germination and Heat Resistance ofFoods (Basel, Switzerland) · 2025Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
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- Hydration solids.Nature · 2023Article
- New Thoughts on an Old Topic: Secrets of Bacterial Spore Resistance Slowly Being Revealed.Microbiology and molecular biology reviews : MMBR · 2023Review
- Bacterial spore germination receptors are nutrient-gated ion channels.Science (New York, N.Y.) · 2023Article
- Expression of the 2Duf protein in wild-type Bacillus subtilis spores stabilizes inner membrane proteins and increases spore resistance to wet heat and hydrogen peroxide.Journal of applied microbiology · 2023Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Insights into the Structure and Protein Composition ofInternational journal of molecular sciences · 2022Article
- Extracellular DNA, cell surface proteins and c-di-GMP promote biofilm formation in Clostridioides difficile.Scientific reports · 2021Article
- Analysis of disulphide bond linkage between CoA and protein cysteine thiols during sporulation and in spores of Bacillus species.FEMS microbiology letters · 2020Article
- Visualization of Germination Proteins in PutativeInternational journal of molecular sciences · 2020Article
- The molecular dynamics of bacterial spore and the role of calcium dipicolinate in core properties at the sub-nanosecond time-scale.Scientific reports · 2020Article
- DNA Damage Kills Bacterial Spores and Cells Exposed to 222-Nanometer UV Radiation.Applied and environmental microbiology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Spores of Bacillus subtilis have a thick outer layer of relatively insoluble protein called the coat, which protects spores against a number of treatments and may also play roles in spore germination. However, elucidation of precise roles of the coat in spore properties has been hampered by the inability to prepare spores lacking all or most coat material. In this work, we show that spores of a strain with mutations in both the cotE and gerE genes, which encode proteins involved in coat assembly and expression of genes encoding coat proteins, respectively, lack most extractable coat protein as seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as well as the great majority of the coat as seen by atomic force microscopy. However, the cotE gerE spores did retain a thin layer of insoluble coat material that was most easily seen by microscopy following digestion of these spores with lysozyme. These severely coat-deficient spores germinated relatively normally with nutrients and even better with dodecylamine but not with a 1:1 chelate of Ca(2+) and dipicolinic acid. These spores were also quite resistant to wet heat, to mechanical disruption, and to treatment with detergents at an elevated temperature and pH but were exquisitely sensitive to killing by sodium hypochlorite. These results provide new insight into the role of the coat layer in spore properties.
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