ArticleInsect biochemistry and molecular biology2008
Transgene expression and silencing in a tick cell line: A model system for functional tick genomics.
Article in Insect biochemistry and molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Genome-wide CRISPR knockout cell screening platform for the disease vector tick speciesbioRxiv : the preprint server for biology · 2026Article
- Validation of heat-inducibleiScience · 2024Article
- Genetic manipulation of anmBio · 2024Article
- Cas9-mediated gene editing in the black-legged tick,iScience · 2022Article
- Alterations in arthropod and neuronal exosomes reduce virus transmission and replication in recipient cells.Extracellular vesicles and circulating nucleic acids · 2022Article
- Acquired tick resistance: The trail is hot.Parasite immunology · 2021Review
- Genetic Manipulation of Ticks: A Paradigm Shift in Tick and Tick-Borne Diseases Research.Frontiers in cellular and infection microbiology · 2021Review
- Karyotype changes in long-term cultured tick cell lines.Scientific reports · 2020Article
- Ixodes scapularis Src tyrosine kinase facilitates Anaplasma phagocytophilum survival in its arthropod vector.Ticks and tick-borne diseases · 2019Article
- The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.Ticks and tick-borne diseases · 2018Review
- Changes in the Proteome of Langat-Infected Ixodes scapularis ISE6 Cells: Metabolic Pathways Associated with Flavivirus Infection.PLoS neglected tropical diseases · 2016Article
- Transgene expression in tick cells using Agrobacterium tumefaciens.Experimental & applied acarology · 2015Article
- An Ixodes scapularis cell line with a predominantly neuron-like phenotype.Experimental & applied acarology · 2015Article
- Motility characteristics are altered for Rickettsia bellii transformed to overexpress a heterologous rickA gene.Applied and environmental microbiology · 2014Article
- Gene silencing in tick cell lines using small interfering or long double-stranded RNA.Experimental & applied acarology · 2013Article
- Vaccination against Lyme disease: past, present, and future.Frontiers in cellular and infection microbiology · 2013Review
- Ixodes scapularis salivary gland protein P11 facilitates migration of Anaplasma phagocytophilum from the tick gut to salivary glands.EMBO reports · 2011Article
- Genetic transformation of the codling moth, Cydia pomonella L., with piggyBac EGFP.Transgenic research · 2011Article
- Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line.Microbiology (Reading, England) · 2010Article
- Evidence of a tick RNAi pathway by comparative genomics and reverse genetics screen of targets with known loss-of-function phenotypes in Drosophila.BMC molecular biology · 2009Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 3 countries.
Funding
Abstract
The genome project of the black legged tick, Ixodes scapularis, provides sequence data for testing gene function and regulation in this important pathogen vector. We tested Sleeping Beauty (SB), a Tc1/mariner group transposable element, and cationic lipid-based transfection reagents for delivery and genomic integration of transgenes into I. scapularis cell line ISE6. Plasmid DNA and dsRNA were effectively transfected into ISE6 cells and they were successfully transformed to express a red fluorescent protein (DsRed2) and a selectable marker, neomycin phosphotransferase (NEO). Frequency of transformation was estimated as 1 transformant per 5000-10,000 cells and cultures were incubated for 2-3 months in medium containing the neomycin analog G418 in order to isolate transformants. Genomic integration of the DsRed2 transgene was confirmed by inverse PCR and sequencing that demonstrated a TA nucleotide pair inserted between SB inverted/direct repeat sequences and tick genomic sequences, indicating that insertion of the DsRed2 gene into the tick cell genome occurred through the activity of SB transposase. RNAi using dsRNA transcribed from the DsRed2 gene silenced expression of red fluorescent protein in transformed ISE6 cells. SB transposition in cell line ISE6 provides an effective means to explore the functional genomics of I. scapularis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.