ArticleThe Journal of urology2008
Directed differentiation of bone marrow derived mesenchymal stem cells into bladder urothelium.
Article in The Journal of urology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
- Exploring better methods for treating urethral stricture caused by lichen sclerosus.Asian journal of andrology · 2026Review
- Mitochondrial Fusion Protein 2-Modified Bone Marrow Mesenchymal Stem Cells Improved Hyperglycemia-Induced Schwann Cell Injury via Regulating Mitochondria-Associated Endoplasmic Reticulum Membranes.Neurourology and urodynamics · 2025Article
- Strategies of Bladder Reconstruction after Partial or Radical Cystectomy for Bladder Cancer.Molecular biotechnology · 2025Review
- Enhanced Bladder Regeneration with Adipose-Derived Stem Cell-Seeded Silk Fibroin Scaffolds: A Comparative Analysis.Biomimetics (Basel, Switzerland) · 2025Article
- Therapeutic effect of integrin-linked kinase gene-modified bone marrow-derived mesenchymal stem cells for streptozotocin-induced diabetic cystopathy in a rat model.Stem cell research & therapy · 2020Article
- Review
- Regenerative and engineered options for urethroplasty.Nature reviews. Urology · 2019Review
- The Therapeutic Potential of Mesenchymal Stromal Cells in the Treatment of Chemotherapy-Induced Tissue Damage.Stem cell reviews and reports · 2019Review
- Mouse and human urothelial cancer organoids: A tool for bladder cancer research.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- In vivo replacement of damaged bladder urothelium by Wolffian duct epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Stem Cells in Functional Bladder Engineering.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2016Review
- Recent advances in ureteral tissue engineering.Current urology reports · 2015Review
- Urine-derived stem cells for potential use in bladder repair.Stem cell research & therapy · 2014Review
- Treatment of bladder dysfunction using stem cell or tissue engineering technique.Korean journal of urology · 2014Review
- Article
- [Stem cell therapy and tissue engineering in regenerative urology].Der Urologe. Ausg. A · 2013Review
- Regenerative medicine based applications to combat stress urinary incontinence.World journal of stem cells · 2013Review
- Article
- Tissue engineering for the oncologic urinary bladder.Nature reviews. Urology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
purposeWe have previously reported that embryonic rat bladder mesenchyma has the appropriate inductive signals to direct pluripotent mouse embryonic stem cells toward endodermal derived urothelium and develop mature bladder tissue. We determined whether nonembryonic stem cells, specifically bone marrow derived mesenchymal stem cells, could serve as a source of pluripotent or multipotent progenitor cells. MATERIALS AND
methodsEpithelium was separated from the mesenchymal shells of embryonic day 14 rat bladders. Mesenchymal stem cells were isolated from mouse femoral and tibial bone marrow. Heterospecific recombinant xenografts were created by combining the embryonic rat bladder mesenchyma shells with mesenchymal stem cells and grafting them into the renal subcapsular space of athymic nude mice. Grafts were harvested at time points of up to 42 days and stained for urothelial and stromal differentiation.
resultsHistological examination of xenografts comprising mouse mesenchymal stem cells and rat embryonic rat bladder mesenchyma yielded mature bladder structures showing normal microscopic architecture as well as proteins confirming functional characteristics. Specifically the induced urothelium expressed uroplakin, a highly selective marker of urothelial differentiation. These differentiated bladder structures demonstrated appropriate alpha-smooth muscle actin staining. Finally, Hoechst staining of the xenografts revealed nuclear architecture consistent with a mouse mesenchymal stem cell origin of the urothelium, supporting differentiated development of these cells.
conclusionsIn the appropriate signaling environment bone marrow derived mesenchymal stem cells can undergo directed differentiation toward endodermal derived urothelium and develop into mature bladder tissue in a tissue recombination model. This model serves as an important tool for the study of bladder development with long-term application toward cell replacement therapies in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.