ArticleFly
Distinct heparan sulfate compositions in wild-type and pipe-mutant eggshell matrix.
Article in Fly. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates.Glycoconjugate journal · 2020Review
- Distinct functional specificities are associated with protein isoforms encoded by the Drosophila dorsal-ventral patterning gene pipe.Development (Cambridge, England) · 2009Article
- Sulfation of eggshell components by Pipe defines dorsal-ventral polarity in the Drosophila embryo.Current biology : CB · 2009Article
- Maternal control of the Drosophila dorsal-ventral body axis.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Spatial information embedded in the extracellular matrix establishes the dorsoventral polarity of the Drosophila embryo through the ventral activity of a serine protease cascade. Pipe is a Golgi-localized protein responsible for generating this spatial information during oogenesis through sulfation of unknown glycans. Although Pipe has sequence homology to glycosaminoglycan 2-O-sulfotransferases, its activity and authentic substrates have not been demonstrated and genetic evidence has argued against a role for glycosaminoglycans in dorsoventral polarity establishment. Here, direct examination of matrix glycosaminoglycans demonstrates that pipe-mutant matrix shows decreased tri-sulfated heparan sulfate compared to wild-type matrix, with correspondingly increased 2-O-sulfated heparan sulfate. Chondroitin sulfate was not detected in this matrix. These results suggest that Pipe promotes 6-O- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-O-sulfation. We discuss the possible significance of these unexpected findings and how they might be reconciled with the genetic data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.