ArticleProceedings of the National Academy of Sciences of the United States of America2008
Mass spectrometry-based discovery of circadian peptides.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 109 citations in OpenAlex.
- Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells.ACS chemical neuroscience · 2026Article
- Temporal control of eating and body weight by GHSR-expressing suprachiasmatic nucleus neurons.Cell reports · 2025Article
- Biological Fluid Flows: Signaling Mediums for Circadian Timing.Journal of biological rhythms · 2025Review
- Article
- The Suprachiasmatic Nucleus at 50: Looking Back, Then Looking Forward.Journal of biological rhythms · 2024Review
- Microanalytical Mass Spectrometry with Super-Resolution Microscopy Reveals a Proteome Transition During Development of the Brain's Circadian Pacemaker.Analytical chemistry · 2023Article
- Perspectives of colon-specific drug delivery in the management of morning symptoms of rheumatoid arthritis.Inflammopharmacology · 2023Review
- Microenvironmental Analysis and Control for Local Cells under Confluent Conditions via a Capillary-Based Microfluidic Device.Analytical chemistry · 2022Article
- Mice lacking proSAAS display alterations in emotion, consummatory behavior and circadian entrainment.Genes, brain, and behavior · 2022Article
- Atomic view of an amyloid dodecamer exhibiting selective cellular toxic vulnerability in acute brain slices.Protein science : a publication of the Protein Society · 2022Article
- Identifying Receptors for Neuropeptides and Peptide Hormones: Challenges and Recent Progress.ACS chemical biology · 2021Review
- Novel approaches leading towards peptide GPCR de-orphanisation.British journal of pharmacology · 2020Review
- Secreted Chaperones in Neurodegeneration.Frontiers in aging neuroscience · 2020Review
- Article
- Neuropeptide PEN and Its Receptor GPR83: Distribution, Signaling, and Regulation.ACS chemical neuroscience · 2019Review
- Functional Peptidomics: Stimulus- and Time-of-Day-Specific Peptide Release in the Mammalian Circadian Clock.ACS chemical neuroscience · 2018Article
- Peptide identifications and false discovery rates using different mass spectrometry platforms.Talanta · 2018Article
- Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees.Advances in neurobiology · 2018Article
- Improved identification and quantitation of mature endogenous peptides in the rodent hypothalamus using a rapid conductive sample heating system.The Analyst · 2017Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
A significant challenge to understanding dynamic and heterogeneous brain systems lies in the chemical complexity of secreted intercellular messengers that change rapidly with space and time. Two solid-phase extraction collection strategies are presented that relate time and location of peptide release with mass spectrometric characterization. Here, complex suites of peptide-based cell-to-cell signaling molecules are characterized from the mammalian suprachiasmatic nucleus (SCN), site of the master circadian clock. Observed SCN releasates are peptide rich and demonstrate the co-release of established circadian neuropeptides and peptides with unknown roles in circadian rhythms. Additionally, the content of SCN releasate is stimulation specific. Stimulation paradigms reported to alter clock timing, including electrical stimulation of the retinohypothalamic tract, produce releasate mass spectra that are notably different from the spectra of compounds secreted endogenously over the course of the 24-h cycle. In addition to established SCN peptides, we report the presence of proSAAS peptides in releasates. One of these peptides, little SAAS, exhibits robust retinohypothalamic tract-stimulated release from the SCN, and exogenous application of little SAAS induces a phase delay consistent with light-mediated cues regulating circadian timing. These mass spectrometry-based analyses provide a new perspective on peptidergic signaling within the SCN and demonstrate that the integration of secreted compounds with information relating time and location of release generates new insights into intercellular signaling in the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.