ArticleProceedings of the National Academy of Sciences of the United States of America2008
The Kv channel blocker 4-aminopyridine enhances Ag+ uptake: a scanning electrochemical microscopy study of single living cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Review
- Nanoelectrochemistry at liquid/liquid interfaces for analytical, biological, and material applications.Chemical communications (Cambridge, England) · 2023Review
- Mechanistic Assessment of Metabolic Interaction between Single Oral Commensal Cells by Scanning Electrochemical Microscopy.Analytical chemistry · 2023Article
- Can AgThe journal of physical chemistry letters · 2023Article
- Scanning electrochemical microscopy and its potential for studying biofilms and antimicrobial coatings.Analytical and bioanalytical chemistry · 2020Review
- Dielectric-dependent electron transfer behaviour of cobalt hexacyanides in a solid solution of sodium chloride.Chemical science · 2015Article
- Ion permeability of the nuclear pore complex and ion-induced macromolecular permeation as studied by scanning electrochemical and fluorescence microscopy.Analytical chemistry · 2014Article
- Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Ion-selective permeability of an ultrathin nanoporous silicon membrane as probed by scanning electrochemical microscopy using micropipet-supported ITIES tips.Analytical chemistry · 2010Article
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3 authors.
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Abstract
We report that silver ion (Ag(+)) uptake is enhanced by 4-aminopyridine (4-AP), a well known voltage-sensitive potassium ion channel (K(v)) blocker. Both bacterial (Escherichia coli) and mammalian (3T3 fibroblast) cells were used as model systems. Ag(+) uptake was monitored with a scanning electrochemical microscope with an amperometric Ag(+) ion-selective electrode (Ag(+)-ISE) and the respiration rates of E. coli cells were measured by oxygen reduction at an ultramicroelectrode. The results showed that not only the amount but also the rate of silver uptake by the cells increased significantly when 4-AP was added to the solution. For fibroblasts, the Ag(+) uptake rate was 4.8 x 10(7) ions per cell per sec without 4-AP compared with 1.0 x 10(8) ions per cell per sec with 0.2 mM 4-AP. For E. coli cells, the uptake rate was 1.5 x 10(4) ions per cell per sec without 4-AP vs. 3.5 x 10(4) ions per cell per sec with 0.5 mM 4-AP and 5.9 x 10(4) ions per cell per sec with 1 mM 4-AP. Thus, 4-AP might be useful where silver is used as antimicrobial agent to speed its uptake.
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