ArticleProceedings of the National Academy of Sciences of the United States of America2008
Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 139 citations in OpenAlex.
- Surface Density-Dependent Interactions between Photoactivated Sensory Rhodopsin 2 and Its Transducer.ACS omega · 2026Article
- Plasmonic-Enhanced Infrared Absorption Platform for Broadband and Multiple Molecular Fingerprint Retrieval.Nanomaterials (Basel, Switzerland) · 2025Article
- An Infrared Nanospectroscopy Technique for the Study of Electric-Field-Induced Molecular Dynamics.Nano letters · 2024Article
- Development and Characterization of Electrodes for Surface-Specific Attenuated Total Reflection Two-Dimensional Infrared Spectroelectrochemistry.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023Article
- Research Progress in Surface-Enhanced Infrared Absorption Spectroscopy: From Performance Optimization, Sensing Applications, to System Integration.Nanomaterials (Basel, Switzerland) · 2023Review
- Probing Denaturation of Protein A via Surface-Enhanced Infrared Absorption Spectroscopy.Biosensors · 2022Article
- Monitoring the Progression of Cell-Free Expression of Microbial Rhodopsins by Surface Enhanced IR Spectroscopy: Resolving a Branch Point for Successful/Unsuccessful Folding.Frontiers in molecular biosciences · 2022Article
- Vibrational spectroscopy analysis of ligand efficacy in human MCommunications biology · 2021Article
- Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy.Lab on a chip · 2021Article
- Is the Use of Surface-Enhanced Infrared Spectroscopy Justified in the Selection of Peptide Fragments That Play a Role in Substrate-Receptor Interactions? Adsorption of Amino Acids and Neurotransmitters on Colloidal Ag and Au Nanoparticles.The journal of physical chemistry. B · 2021Article
- Structure Changes of a Membrane Polypeptide under an Applied Voltage Observed with Surface-Enhanced 2D IR Spectroscopy.The journal of physical chemistry letters · 2021Article
- Near-Infrared Activation of Sensory Rhodopsin II Mediated by NIR-to-Blue Upconversion Nanoparticles.Frontiers in molecular biosciences · 2021Article
- Label-free Optical Imaging of Membrane Potential.Current opinion in biomedical engineering · 2019Article
- Dynamic surface properties of PEG-coated CuS nanoparticles alter their interaction with cells as revealed by surface-enhanced infrared spectroscopy.Nanoscale advances · 2019Article
- Tip-Enhanced Infrared Difference-Nanospectroscopy of the Proton Pump Activity of Bacteriorhodopsin in Single Purple Membrane Patches.Nano letters · 2019Article
- Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase.Journal of visualized experiments : JoVE · 2017Article
- pH and Potential Transients of the bcThe journal of physical chemistry. B · 2017Article
- Biomimetic Membranes for Multi-Redox Center Proteins.International journal of molecular sciences · 2016Review
- Time-Resolved Proteomic Visualization of Dendrimer Cellular Entry and Trafficking.Journal of the American Chemical Society · 2015Article
- Specific interactions between alkali metal cations and the KcsA channel studied using ATR-FTIR spectroscopy.Biophysics and physicobiology · 2015Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane proteins are molecular machines that transport ions, solutes, or information across the cell membrane. Electrophysiological techniques have unraveled many functional aspects of ion channels but suffer from the lack of structural sensitivity. Here, we present spectroelectrochemical data on vibrational changes of membrane proteins derived from a single monolayer. For the seven-helical transmembrane protein sensory rhodopsin II, structural changes of the protein backbone and the retinal cofactor as well as single ion transfer events are resolved by surface-enhanced IR difference absorption spectroscopy (SEIDAS). Angular changes of bonds versus the membrane normal have been determined because SEIDAS monitors only those vibrations whose dipole moment are oriented perpendicular to the solid surface. The application of negative membrane potentials (DeltaV = -0.3 V) leads to the selective halt of the light-induced proton transfer at the stage of D75, the counter ion of the retinal Schiff base. It is inferred that the voltage raises the energy barrier of this particular proton-transfer reaction, rendering the energy deposited in the retinal by light excitation insufficient for charge transfer to occur. The other structural rearrangements that accompany light-induced activity of the membrane protein, are essentially unaffected by the transmembrane electric field. Our results demonstrate that SEIDAS is a generic approach to study processes that depend on the membrane potential, like those in voltage-gated ion channels and transporters, to elucidate the mechanism of ion transfer with unprecedented spatial sensitivity and temporal resolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.