ArticleProceedings of the National Academy of Sciences of the United States of America2008
TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 192 citations in OpenAlex.
- Phosgene inhalation injury in mice: Modeling human pathophysiology, biomarker discovery, and therapeutic target identification.Toxicology and applied pharmacology · 2026Article
- Cigarette smoke extract (CSE) reduces expression of functional TRPV4 channels in primary human bronchial epithelial cells differentiated at an air liquid interface (ALI) in vitro.Archives of toxicology · 2026Article
- Extrinsic Modulation of the Zonular Tension Induces Calcium Influx in Lens Fiber Cells That Is Mediated by Mechanosensitive TRPV1 and TRPV4 Channels.Investigative ophthalmology & visual science · 2026Article
- Transient receptor potential vanilloid 4 (TRPV4) channels mediate pulmonary surfactant protein A and D secretion.American journal of respiratory cell and molecular biology · 2026Article
- Transient receptor potential vanilloid 4 channels contribute to the initiation of water-induced swallowing reflexes.Scientific reports · 2026Article
- Transient receptor potential vanilloid 4 channels contribute to the initiation of water-induced swallowing reflexes.Research square · 2026Article
- Regional Differences in Calcium Dynamics in the Intact Lens: The Role of TRPV Channels in Lens Calcium Regulation.Investigative ophthalmology & visual science · 2025Article
- TRPV4 Channel Modulators as Potential Drug Candidates for Cystic Fibrosis.International journal of molecular sciences · 2024Review
- Identification and Properties of TRPV4 Mutant Channels Present in Polycystic Kidney Disease Patients.Function (Oxford, England) · 2024Article
- Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination.Nature communications · 2024Article
- Calcium Signaling in Airway Epithelial Cells: Current Understanding and Implications for Inflammatory Airway Disease.Arteriosclerosis, thrombosis, and vascular biology · 2024Review
- Ambroxol-Enhanced Frequency and Amplitude of Beating Cilia Controlled by a Voltage-Gated CaInternational journal of molecular sciences · 2023Article
- An estimation of osteochondrodysplasia prevalence in Australian Scottish Fold cats: a retrospective study using VetCompass Data.BMC veterinary research · 2023Article
- Ouabain's Influence on TRPV4 Channels of Epithelial Cells: An Exploration of TRPV4 Activity, Expression, and Signaling Pathways.International journal of molecular sciences · 2023Article
- Activation of renal vascular smooth muscle TRPV4 channels by 5-hydroxytryptamine impairs kidney function in neonatal pigs.Microvascular research · 2023Article
- Unconventional interactions of the TRPV4 ion channel with beta-adrenergic receptor ligands.Life science alliance · 2023Article
- Mechanosensitive channels in lung disease.Frontiers in physiology · 2023Review
- Oxygen and mechanical stretch in the developing lung: risk factors for neonatal and pediatric lung disease.Frontiers in medicine · 2023Review
- Pharmacological activation of transient receptor potential vanilloid 4 promotes triggering of the swallowing reflex in rats.Frontiers in cellular neuroscience · 2023Article
- Article
44 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The rate of mucociliary clearance in the airways is a function of ciliary beat frequency (CBF), and this, in turn, is increased by increases in intracellular calcium. The TRPV4 cation channel mediates Ca(2+) influx in response to mechanical and osmotic stimuli in ciliated epithelia. With the use of a TRPV4-deficient mouse, we now show that TRPV4 is involved in the airways' response to physiologically relevant physical and chemical stimuli. Ciliary TRPV4 expression in tracheal epithelial cells was confirmed with immunofluorescence in TRPV4(+/+) mice. Ciliated tracheal cells from TRPV4(-/-) mice showed no increases in intracellular Ca(2+) and CBF in response to the synthetic activator 4alpha-phorbol 12,13-didecanoate (4alphaPDD) and reduced responses to mild temperature, another TRPV4-activating stimulus. Autoregulation of CBF in response to high viscosity solutions is preserved in TRPV4(-/-) despite a reduced Ca(2+) signal. More interestingly, TRPV4 contributed to an ATP-induced increase in CBF, providing a pathway for receptor-operated Ca(2+) entry but not store-operated Ca(2+) entry as the former mechanism is lost in TRPV4(-/-) cells. Collectively, these results suggest that TRPV4 is predominantly located in the cilia of tracheal epithelial cells and plays a key role in the transduction of physical and chemical stimuli into a Ca(2+) signal that regulates CBF and mucociliary transport. Moreover, these studies implicate the participation of TRPV4 in receptor-operated Ca(2+) entry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.