ArticleCellular microbiology2008
Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells.
Article in Cellular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Bacterial extracellular vesicles - brain invaders? A systematic review.Frontiers in molecular neuroscience · 2023Pooled it
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- Dysregulation of Stress-Induced Translational Control byMicroorganisms · 2023Article
- Local and Systemic Effects ofMicroorganisms · 2023Review
- Superparamagnetic Iron Oxide Nanoparticles Protect Human Gingival Fibroblasts fromInternational journal of nanomedicine · 2022Article
- Shut-Down of Type IX Protein Secretion Alters the Host Immune Response toFrontiers in cellular and infection microbiology · 2022Article
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- Outer Membrane Vesicles (OMVs) of Gram-negative Bacteria: A Perspective Update.Frontiers in microbiology · 2017Review
- Mechanisms of outer membrane vesicle entry into host cells.Cellular microbiology · 2016Review
- Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells.PloS one · 2016Article
- Article
- Transglutaminase 2 is essential for adherence of Porphyromonas gingivalis to host cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Francisella tularensis uses cholesterol and clathrin-based endocytic mechanisms to invade hepatocytes.Scientific reports · 2011Article
- Translocation of Porphyromonas gingivalis gingipain adhesin peptide A44 to host mitochondria prevents apoptosis.Infection and immunity · 2010Article
- Bacterial invasion of epithelial cells and spreading in periodontal tissue.Periodontology 2000 · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Porphyromonas gingivalis, a Gram-negative oral anaerobe, is associated with periodontitis, a disease that in some form affects up to 80% of the adult population in the USA. The organism interacts with gingival epithelium and surrounding tissue, and in this study we analysed interactions initiated by P. gingivalis and by a peptide derived from the adhesin domain of arg-gingipain A, a member of a family of surface cysteine proteinases. Recombinant peptide A44 blocked adherence of bacteria to host cell monolayers, and bound to components of the cell membrane fraction. In pull-down assays A44 associated with proteins involved in a clathrin-dependent endocytosis pathway. Inhibitor studies confirmed a role for clathrin, and confocal microscopy demonstrated that both A44-coated beads and intact bacteria colocalized with GFP-clathrin in host cells. Finally, we used siRNA to determine whether clathrin or caveolin-1 was involved in association of peptide and intact bacteria with host cells. Again, the results of these assays indicated that association of both A44 and P. gingivalis depended on the presence of clathrin, and support a working model in which A44 initiates a clathrin-dependent pathway that potentially leads to internalization of peptide or bacteria by host epithelial cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.