ArticleBiochemistry2008
Thioredoxin reductase from Thermoplasma acidophilum: a new twist on redox regulation.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Unlocking Survival Mechanisms for Metal and Oxidative Stress in the Extremely Acidophilic, HalotolerantGenes · 2020Article
- Crystal Structure of the Apo-Form of NADPH-Dependent Thioredoxin Reductase from a Methane-Producing Archaeon.Antioxidants (Basel, Switzerland) · 2018Article
- Unprecedented pathway of reducing equivalents in a diflavin-linked disulfide oxidoreductase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Reexamination of Thioredoxin Reductase fromACS omega · 2017Article
- Methanosarcina acetivorans utilizes a single NADPH-dependent thioredoxin system and contains additional thioredoxin homologues with distinct functions.Microbiology (Reading, England) · 2017Article
- Biochemical Function, Molecular Structure and Evolution of an Atypical Thioredoxin Reductase fromFrontiers in microbiology · 2017Article
- A Novel F420-dependent Thioredoxin Reductase Gated by Low Potential FAD: A TOOL FOR REDOX REGULATION IN AN ANAEROBE.The Journal of biological chemistry · 2016Article
- The structural basis of an NADP⁺-independent dithiol oxidase in FK228 biosynthesis.Scientific reports · 2014Article
- Thioredoxin targets fundamental processes in a methane-producing archaeon, Methanocaldococcus jannaschii.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Properties of the endogenous components of the thioredoxin system in the psychrophilic eubacterium Pseudoalteromonas haloplanktis TAC 125.Extremophiles : life under extreme conditions · 2012Article
- Reactivity of thioredoxin as a protein thiol-disulfide oxidoreductase.Chemical reviews · 2011Review
- Structure of Hordeum vulgare NADPH-dependent thioredoxin reductase 2. Unwinding the reaction mechanism.Acta crystallographica. Section D, Biological crystallography · 2009Article
- Direct electrochemical analyses of a thermophilic thioredoxin reductase: interplay between conformational change and redox chemistry.Biochemistry · 2008Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Thioredoxin reductases (TrxRs) regulate the intracellular redox environment by using NADPH to provide reducing equivalents for thioredoxins (Trxs). Here we present the cloning and biochemical characterization of a putative TrxR (Ta0984) and a putative Trx (Ta0866) from Thermoplasma acidophilum. Our data identify Ta0866 as a Trx through its capacity to reduce insulin and be reduced by Escherichia coli TrxR in a NADPH-dependent manner. Our data also establish Ta0984 as a TrxR due to its ability to reduce T. acidophilum Trx ( taTrx), although not in a NADPH- or NADH-dependent manner. To explore the apparent inability of taTrxR to use NADPH or NADH as a reductant, we carried out a complete electrochemical characterization, which suggests that redox potential is not the source of this nonreactivity [Hamill et al. (2008) Biochemistry 47, 9738-9746]. Turning to crystallographic analysis, a 2.35 A resolution structure of taTrxR, also presented here, shows that despite the overall structural similarity to the well-characterized TrxR from E. coli (RMSD 1.30 A (2) for chain A), the "NADPH binding pocket" is not conserved. E. coli TrxR residues implicated in NADPH binding, H175, R176, R177, and R181, have been substituted with E185, Y186, M187, and M191 in the ta protein. Thus, we have identified a Trx and TrxR protein system from T. acidophilum for which the TrxR shares overall structural and redox properties with other TrxRs but lacks the appropriate binding motif to use the standard NADPH reductant. Our discovery of a TrxR that does not use NADPH provides a new twist in redox regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.