ArticlePflugers Archiv : European journal of physiology2009
Cultured ruminal epithelial cells express a large-conductance channel permeable to chloride, bicarbonate, and acetate.
Article in Pflugers Archiv : European journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Genome-Wide Identification of Reference Genes for Reverse-Transcription Quantitative PCR in Goat Rumen.Animals : an open access journal from MDPI · 2021Article
- The ATP-Releasing Maxi-Cl Channel: Its Identity, Molecular Partners and Physiological/Pathophysiological Implications.Life (Basel, Switzerland) · 2021Review
- Diet Transition from High-Forage to High-Concentrate Alters Rumen Bacterial Community Composition, Epithelial Transcriptomes and Ruminal Fermentation Parameters in Dairy Cows.Animals : an open access journal from MDPI · 2021Article
- Butyrate Permeation across the Isolated Ovine Reticulum Epithelium.Animals : an open access journal from MDPI · 2020Article
- Effect of individual SCFA on the epithelial barrier of sheep rumen under physiological and acidotic luminal pH conditions.Journal of animal science · 2018Article
- Evidence for the functional involvement of members of the TRP channel family in the uptake of Na(+) and NH4 (+) by the ruminal epithelium.Pflugers Archiv : European journal of physiology · 2016Article
- The properties, functions, and pathophysiology of maxi-anion channels.Pflugers Archiv : European journal of physiology · 2016Review
- Short-term adaptation of the ruminal epithelium involves abrupt changes in sodium and short-chain fatty acid transport.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Epithelia of the ovine and bovine forestomach express basolateral maxi-anion channels permeable to the anions of short-chain fatty acids.Pflugers Archiv : European journal of physiology · 2014Article
- Effects of dietary fibre and protein on urea transport across the cecal mucosa of piglets.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2013Article
- The maxi-anion channel: a classical channel playing novel roles through an unidentified molecular entity.The journal of physiological sciences : JPS · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The absorption of short-chain fatty acids (SCFA) from the rumen requires efficient mechanisms for both apical uptake and basolateral extrusion. Previous studies suggest that the rumen expresses a basolateral chloride conductance that might be permeable to SCFA. In order to characterize this conductance in more detail, isolated cultured ruminal epithelial cells were studied with the patch-clamp technique, revealing a whole-cell conductance with p(Cl(-)) approximately p(NO(3) (-)) > p(HCO(3) (-)) > p(acetate(-)) > p(gluconate(-)). Currents could be blocked by diisothiocyanato-stilbene-2,2'-disulfonic acid (1 mmol l(-1) > 100 micromol l(-1)), 5-nitro-2-(3-phenylpropyl-amino)benzoic acid (50 micromol l(-1)), niflumic acid (100 micromol l(-1)), and p-chloromercuribenzoate (1 mmol l(-1)). Single-channel conductance was 350 +/- 7 pS for chloride and 142 +/- 7 pS for acetate. Open probability could be fitted with a three-state gating model. We propose a role for this channel in mediating the permeation of chloride, bicarbonate, and acetate across the basolateral membrane of the ruminal epithelium.
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