ArticlePloS one2008
Gene expression and functional studies of the optic nerve head astrocyte transcriptome from normal African Americans and Caucasian Americans donors.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Genetic risk factors associated with ocular perfusion pressure in primary open-angle glaucoma.Human genomics · 2025Article
- Major review: Molecular genetics of primary open-angle glaucoma.Experimental eye research · 2017Review
- Role of cyclic AMP in the eye with glaucoma.BMB reports · 2017Review
- Outcome Disparities in African American Compared with European American Women with ER+HER2- Tumors Treated within an Equal-Access Health Care System.Ethnicity & disease · 2016Article
- Effect of obesity on molecular characteristics of invasive breast tumors: gene expression analysis in a large cohort of female patients.BMC obesity · 2016Article
- Isolation of intact astrocytes from the optic nerve head of adult mice.Experimental eye research · 2015Article
- Differential gene expression in glaucoma.Cold Spring Harbor perspectives in medicine · 2014Review
- Human GGT2 does not autocleave into a functional enzyme: A cautionary tale for interpretation of microarray data on redox signaling.Antioxidants & redox signaling · 2013Article
- Involvement of EphB/Ephrin-B signaling in axonal survival in mouse experimental glaucoma.Investigative ophthalmology & visual science · 2012Article
- The immune response in glaucoma: a perspective on the roles of oxidative stress.Experimental eye research · 2011Review
- Characterization of the proteome, diseases and evolution of the human postsynaptic density.Nature neuroscience · 2011Article
- Modulation of factors affecting optic nerve head astrocyte migration.Investigative ophthalmology & visual science · 2010Article
- Gene expression changes in areas of focal loss of retinal ganglion cells in the retina of DBA/2J mice.Investigative ophthalmology & visual science · 2010Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
purposeTo determine whether optic nerve head (ONH) astrocytes, a key cellular component of glaucomatous neuropathy, exhibit differential gene expression in primary cultures of astrocytes from normal African American (AA) donors compared to astrocytes from normal Caucasian American (CA) donors.
methodsWe used oligonucleotide Affymetrix microarray (HG U133A & HG U133A 2.0 chips) to compare gene expression levels in cultured ONH astrocytes from twelve CA and twelve AA normal age matched donor eyes. Chips were normalized with Robust Microarray Analysis (RMA) in R using Bioconductor. Significant differential gene expression levels were detected using mixed effects modeling and Statistical Analysis of Microarray (SAM). Functional analysis and Gene Ontology were used to classify differentially expressed genes. Differential gene expression was validated by quantitative real time RT-PCR. Protein levels were detected by Western blots and ELISA. Cell adhesion and migration assays tested physiological responses. Glutathione (GSH) assay detected levels of intracellular GSH.
resultsMultiple analyses selected 87 genes differentially expressed between normal AA and CA (P<0.01). The most relevant genes expressed in AA were categorized by function, including: signal transduction, response to stress, ECM genes, migration and cell adhesion.
conclusionsThese data show that normal astrocytes from AA and CA normal donors display distinct expression profiles that impact astrocyte functions in the ONH. Our data suggests that differences in gene expression in ONH astrocytes may be specific to the development and/or progression of glaucoma in AA.
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