ArticleMolecular biology of the cell2008
Phosphatase inhibitor-2 balances protein phosphatase 1 and aurora B kinase for chromosome segregation and cytokinesis in human retinal epithelial cells.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 55 citations in OpenAlex.
- Inhibitor-2 directs formation of PP1 holoenzymes through a docking motif-dependent transfer of catalytic subunits to adapters.bioRxiv : the preprint server for biology · 2026Article
- NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody.Protein & cell · 2024Article
- Identification of potential biomarkers of myopia based on machine learning algorithms.BMC ophthalmology · 2023Article
- Regulation of PP1 interaction with I-2, neurabin, and F-actin.Molecular and cellular neurosciences · 2023Article
- Protein phosphatase-1 inhibitor-2 promotes PP1Frontiers in synaptic neuroscience · 2022Article
- The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture.BMC molecular and cell biology · 2020Article
- PP1 promotes cyclin B destruction and the metaphase-anaphase transition by dephosphorylating CDC20.Molecular biology of the cell · 2020Article
- Co-option of Plasmodium falciparum PP1 for egress from host erythrocytes.Nature communications · 2020Article
- Protein phosphatase 1 regulatory subunit 1A regulates cell cycle progression in Ewing sarcoma.Oncotarget · 2020Article
- Protein phosphatases in the regulation of mitosis.The Journal of cell biology · 2019Review
- Signaling Enzymes Required for Sperm Maturation and Fertilization in Mammals.Frontiers in cell and developmental biology · 2019Review
- Successful overexpression of wild-type inhibitor-2 of PP1 in cardiovascular cells.Naunyn-Schmiedeberg's archives of pharmacology · 2018Article
- Bipolar disorder with binge eating behavior: a genome-wide association study implicates PRR5-ARHGAP8.Translational psychiatry · 2018Article
- The Toxoplasma gondii inhibitor-2 regulates protein phosphatase 1 activity through multiple motifs.Parasitology research · 2017Article
- Aurora B expression modulates paclitaxel response in non-small cell lung cancer.British journal of cancer · 2017Article
- Protein Phosphatase 1 Down Regulates ZYG-1 Levels to Limit Centriole Duplication.PLoS genetics · 2017Article
- Not so pseudo: the evolutionary history of protein phosphatase 1 regulatory subunit 2 and related pseudogenes.BMC evolutionary biology · 2013Article
- PPP1R42, a PP1 binding protein, regulates centrosome dynamics in ARPE-19 cells.Biology of the cell · 2013Article
- Plasmodium falciparum encodes a conserved active inhibitor-2 for Protein Phosphatase type 1: perspectives for novel anti-plasmodial therapy.BMC biology · 2013Article
- Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa.BMC cell biology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Mitosis in Saccharomyces cerevisiae depends on IPL1 kinase, which genetically interacts with GLC8. The metazoan homologue of GLC8 is inhibitor-2 (I-2), but its function is not understood. We found endogenous and ectopic I-2 localized to the spindle, midzone, and midbody of mitotic human epithelial ARPE-19 cells. Knockdown of I-2 by RNA interference produced multinucleated cells, with supernumerary centrosomes, multipolar spindles and lagging chromosomes during anaphase. These defects did not involve changes in levels of protein phosphatase-1 (PP1), and the multinuclear phenotype was rescued by overexpression of I-2. Appearance of multiple nuclei and supernumerary centrosomes required progression through the cell cycle and I-2 knockdown cells failed cytokinesis, as observed by time-lapse microscopy. Inhibition of Aurora B by hesperadin produced multinucleated cells and reduced H3S10 phosphorylation. I-2 knockdown enhanced this latter effect. Partial knockdown of PP1Calpha prevented multiple nuclei caused by either knockdown of I-2 or treatment with hesperadin. Expression of enhanced green fluorescent protein-I-2 or hemagglutinin-I-2 made cells resistant to hesperadin. We propose that I-2 acts to enhance Aurora B by inhibiting specific PP1 holoenzymes that dephosphorylate Aurora B substrates necessary for chromosome segregation and cytokinesis. Conserved together throughout eukaryotic evolution, I-2, PP1 and Aurora B function interdependently during mitosis.
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