ArticleMolecular biology of the cell2008
Microtubule-mediated Src tyrosine kinase trafficking in neuronal growth cones.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Neuronal NADPH oxidase is required for neurite regeneration of Aplysia bag cell neurons.Journal of neurochemistry · 2023Article
- Tirbanibulin for Actinic Keratosis: Insights into the Mechanism of Action.Clinical, cosmetic and investigational dermatology · 2022Review
- The microtubule network enables Src kinase interaction with the Na,K-ATPase to generate CaFrontiers in physiology · 2022Article
- Signaling Enzymes and Ion Channels Being Modulated by the Actin Cytoskeleton at the Plasma Membrane.International journal of molecular sciences · 2021Review
- A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones.Molecular biology of the cell · 2019Article
- A low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D.Journal of neural engineering · 2018Article
- Regulation of E-cadherin localization by microtubule targeting agents: rapid promotion of cortical E-cadherin through p130Cas/Src inhibition by eribulin.Oncotarget · 2018Article
- Article
- Src and cortactin promote lamellipodia protrusion and filopodia formation and stability in growth cones.Molecular biology of the cell · 2015Article
- Quantifying mechanical force in axonal growth and guidance.Frontiers in cellular neuroscience · 2015Review
- Targeting and transport: how microtubules control focal adhesion dynamics.The Journal of cell biology · 2012Review
- SRC family kinases are required for limb trajectory selection by spinal motor axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Cortactin colocalizes with filopodial actin and accumulates at IgCAM adhesion sites in Aplysia growth cones.Journal of neuroscience research · 2009Article
- Reverse signaling by glycosylphosphatidylinositol-linked Manduca ephrin requires a SRC family kinase to restrict neuronal migration in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Microtubule and cell contact dependency of ER-bound PTP1B localization in growth cones.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Src family tyrosine kinases are important signaling enzymes in the neuronal growth cone, and they have been implicated in axon guidance; however, the detailed localization, trafficking, and cellular functions of Src kinases in live growth cones are unclear. Here, we cloned two novel Aplysia Src kinases, termed Src1 and Src2, and we show their association with both the plasma membrane and the microtubule cytoskeleton in the growth cone by live cell imaging, immunocytochemistry, and cell fractionation. Activated Src2 is enriched in filopodia tips. Interestingly, Src2-enhanced green fluorescent protein-positive endocytic vesicles and tubulovesicular structures undergo microtubule-mediated movements that are bidirectional in the central domain and mainly retrograde in the peripheral domain. To further test the role of microtubules in Src trafficking in the growth cone, microtubules were depleted with either nocodazole or vinblastine treatment, resulting in an increase in Src2 plasma membrane levels in all growth cone domains. Our data suggest that microtubules regulate the steady-state level of active Src at the plasma membrane by mediating retrograde recycling of endocytosed Src. Expression of constitutively active Src2 results in longer filopodia that protrude from smaller growth cones, implicating Src2 in controlling the size of filopodia and lamellipodia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.