ArticlePlant physiology2008
Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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90 citing papers in PubMed, 185 citations in OpenAlex.
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- Starch synthesis in Brachypodium distachyon endosperm occurs in dynamic, connected amyloplast compartments.The New phytologist · 2026Article
- Plant plastids: from evolutionary origins to functional specialization and organelle interactions.Journal of experimental botany · 2025Review
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- Membrane contact sites between chloroplasts and the pathogen interface underpin plant focal immune responses.The Plant cell · 2025Article
- Autophagy plays a dual role in chromoplast transition and degradation and is essential for fruit coloration and ripening.Autophagy · 2025Article
- Recovery of the cortical chloroplast layer in the green algaFrontiers in plant science · 2025Article
- CHUP1 restricts chloroplast movement and effector-triggered immunity in epidermal cells.The New phytologist · 2024Article
- A tool for live-cell confocal imaging of temperature-dependent organelle dynamics.Microscopy (Oxford, England) · 2024Article
- Chloroplast-actin filaments decide the direction of chloroplast avoidance movement under strong light in Arabidopsis thaliana.Journal of plant research · 2024Article
- Bilirubin Distribution in Plants at the Subcellular and Tissue Levels.Plant & cell physiology · 2024Article
- Stromule Geometry Allows Optimal Spatial Regulation of Organelle Interactions in the Quasi-2D Cytoplasm.Plant & cell physiology · 2024Article
- CHLOROPLAST UNUSUAL POSITIONING 1 is a plant-specific actin polymerization factor regulating chloroplast movement.The Plant cell · 2024Article
- Plastid Transient and Stable Interactions with Other Cell Compartments.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Probing the physiological role of the plastid outer-envelope membrane using the oemiR plasmid collection.G3 (Bethesda, Md.) · 2023Article
- FKF1 Interacts with CHUP1 and Regulates Chloroplast Movement in Arabidopsis.Plants (Basel, Switzerland) · 2023Article
- Chloroplasts in plant cells show active glassy behavior under low-light conditions.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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30 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts change their intracellular distribution in response to light intensity. Previously, we isolated the chloroplast unusual positioning1 (chup1) mutant of Arabidopsis (Arabidopsis thaliana). This mutant is defective in normal chloroplast relocation movement and shows aggregation of chloroplasts at the bottom of palisade mesophyll cells. The isolated gene encodes a protein with an actin-binding motif. Here, we used biochemical analyses to determine the subcellular localization of full-length CHUP1 on the chloroplast outer envelope. A CHUP1-green fluorescent protein (GFP) fusion, which was detected at the outermost part of mesophyll cell chloroplasts, complemented the chup1 phenotype, but GFP-CHUP1, which was localized mainly in the cytosol, did not. Overexpression of the N-terminal hydrophobic region (NtHR) of CHUP1 fused with GFP (NtHR-GFP) induced a chup1-like phenotype, indicating a dominant-negative effect on chloroplast relocation movement. A similar pattern was found in chloroplast OUTER ENVELOPE PROTEIN7 (OEP7)-GFP transformants, and a protein containing OEP7 in place of NtHR complemented the mutant phenotype. Physiological analyses of transgenic Arabidopsis plants expressing truncated CHUP1 in a chup1 mutant background and cytoskeletal inhibitor experiments showed that the coiled-coil region of CHUP1 anchors chloroplasts firmly on the plasma membrane, consistent with the localization of coiled-coil GFP on the plasma membrane. Thus, CHUP1 localization on chloroplasts, with the N terminus inserted into the chloroplast outer envelope and the C terminus facing the cytosol, is essential for CHUP1 function, and the coiled-coil region of CHUP1 prevents chloroplast aggregation and participates in chloroplast relocation movement.
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