ArticlePLoS neglected tropical diseases2008
Entamoeba shows reversible variation in ploidy under different growth conditions and between life cycle phases.
Article in PLoS neglected tropical diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Diverse Genome Structures among Eukaryotes May Have Arisen in Response to Genetic Conflict.Genome biology and evolution · 2024Review
- Article
- Giant cells: multiple cells unite to survive.Frontiers in cellular and infection microbiology · 2023Review
- The evolutionary cancer genome theory and its reasoning.Genetics in medicine open · 2023Review
- Coupling between Ribotypic and Phenotypic Traits of Protists across Life Cycle Stages and Temperatures.Microbiology spectrum · 2021Article
- Encystation ofMicroorganisms · 2021Article
- Entamoeba histolytica: Five facts about modeling a complex human disease in rodents.PLoS pathogens · 2020Article
- Article
- DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia.Parasitology research · 2018Article
- Cellular Events of Multinucleated Giant Cells Formation During the Encystation ofFrontiers in cellular and infection microbiology · 2018Article
- Structure of Ca2+-binding protein-6 from Entamoeba histolytica and its involvement in trophozoite proliferation regulation.PLoS pathogens · 2017Article
- Homologous Recombination in Protozoan Parasites and Recombinase Inhibitors.Frontiers in microbiology · 2017Review
- TheCurrent tropical medicine reports · 2016Article
- Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica.Scientific reports · 2016Article
- Recent advances inF1000Research · 2016Review
- Evolutionary genomics and population structure of Entamoeba histolytica.Computational and structural biotechnology journal · 2014Review
- The macrophage migration inhibitory factor homolog of Entamoeba histolytica binds to and immunomodulates host macrophages.Infection and immunity · 2014Article
- Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.PloS one · 2013Article
- Real-time analysis of gut flora in Entamoeba histolytica infected patients of Northern India.BMC microbiology · 2012Article
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3 authors.
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Abstract
Under axenic growth conditions, trophozoites of Entamoeba histolytica contain heterogenous amounts of DNA due to the presence of both multiple nuclei and different amounts of DNA in individual nuclei. In order to establish if the DNA content and the observed heterogeneity is maintained during different growth conditions, we have compared E. histolytica cells growing in xenic and axenic cultures. Our results show that the nuclear DNA content of E. histolytica trophozoites growing in axenic cultures is at least 10 fold higher than in xenic cultures. Re-association of axenic cultures with their bacterial flora led to a reduction of DNA content to the original xenic values. Thus switching between xenic and axenic growth conditions was accompanied by significant changes in the nuclear DNA content of this parasite. Changes in DNA content during encystation-excystation were studied in the related reptilian parasite E. invadens. During excystation of E. invadens cysts, it was observed that the nuclear DNA content increased approximately 40 fold following emergence of trophozoites in axenic cultures. Based on the observed large changes in nuclear size and DNA content, and the minor differences in relative abundance of representative protein coding sequences, rDNA and tRNA sequences, it appears that gain or loss of whole genome copies may be occurring during changes in the growth conditions. Our studies demonstrate the inherent plasticity and dynamic nature of the Entamoeba genome in at least two species.
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