ArticlePloS one2008
Freeze-dried somatic cells direct embryonic development after nuclear transfer.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.Scientific reports · 2022Article
- Healthy cloned offspring derived from freeze-dried somatic cells.Nature communications · 2022Article
- Molecular and Histological Evaluation of Sheep Ovarian Tissue Subjected to Lyophilization.Animals : an open access journal from MDPI · 2021Article
- Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations.Scientific reports · 2021Article
- Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station.Science advances · 2021Article
- DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development.The Journal of reproduction and development · 2018Article
- Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing.Oncotarget · 2018Article
- Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum.Scientific reports · 2018Article
- High post-thaw survival of ram sperm after partial freeze-drying.Journal of assisted reproduction and genetics · 2018Article
- Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity.Scientific reports · 2017Article
- Production of hand-made cloned buffalo (Bubalus bubalis) embryos from non-viable somatic cells.In vitro cellular & developmental biology. Animal · 2016Article
- Sheep: the first large animal model in nuclear transfer research.Cellular reprogramming · 2013Review
- Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.PloS one · 2013Article
- The role of nuclear technologies in the diagnosis and control of livestock diseases--a review.Tropical animal health and production · 2012Review
- Effect of postmortem time interval on in vitro culture potential of goat skin tissues stored at room temperature.In vitro cellular & developmental biology. Animal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
The natural capacity of simple organisms to survive in a dehydrated state has long been exploited by man, with lyophylization the method of choice for the long term storage of bacterial and yeast cells. More recently, attempts have been made to apply this procedure to the long term storage of blood cells. However, despite significant progress, practical application in a clinical setting is still some way off. Conversely, to date there are no reports of attempts to lyophilize nucleated somatic cells for possible downstream applications. Here we demonstrate that lyophilised somatic cells stored for 3 years at room temperature are able to direct embryonic development following injection into enucleated oocytes. These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine and for conservation strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.