ArticleThe Journal of biological chemistry2008
Phosphorylation of the yeast nitrate transporter Ynt1 is essential for delivery to the plasma membrane during nitrogen limitation.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- The NPR/Hal family of protein kinases in yeasts: biological role, phylogeny and regulation under environmental challenges.Computational and structural biotechnology journal · 2022Review
- Article
- CaNRT2.1 Is Required for Nitrate but Not Nitrite Uptake in Chili Pepper PathogenFrontiers in microbiology · 2020Article
- Genome-wide associated study identifies NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice.Nature communications · 2019Article
- Article
- Molecular components of nitrate and nitrite efflux in yeast.Eukaryotic cell · 2014Article
- Tiered regulation of sulfur deprivation responses in Chlamydomonas reinhardtii and identification of an associated regulatory factor.Plant physiology · 2013Article
- Internal amino acids promote Gap1 permease ubiquitylation via TORC1/Npr1/14-3-3-dependent control of the Bul arrestin-like adaptors.Molecular and cellular biology · 2012Article
- Regulation of high-affinity nitrate uptake in roots of Arabidopsis depends predominantly on posttranscriptional control of the NRT2.1/NAR2.1 transport system.Plant physiology · 2012Article
- Dynamic model of flexible phytoplankton nutrient uptake.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Npr1 Ser/Thr protein kinase links nitrogen source quality and carbon availability with the yeast nitrate transporter (Ynt1) levels.The Journal of biological chemistry · 2011Article
- Role of the Npr1 kinase in ammonium transport and signaling by the ammonium permease Mep2 in Candida albicans.Eukaryotic cell · 2011Article
- Ure2 is involved in nitrogen catabolite repression and salt tolerance via Ca2+ homeostasis and calcineurin activation in the yeast Hansenula polymorpha.The Journal of biological chemistry · 2010Article
- Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences.The Journal of biological chemistry · 2010Article
- Identification and regulation of plasma membrane sulfate transporters in Chlamydomonas.Plant physiology · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ynt1 is the sole high affinity nitrate transporter of the yeast Hansenula polymorpha. It is highly regulated by the nitrogen source, by being down-regulated in response to glutamine by repression of the YNT1 gene and Ynt1 ubiquitinylation, endocytosis, and vacuolar degradation. On the contrary, we show that nitrogen limitation stabilizes Ynt1 levels at the plasma membrane, requiring phosphorylation of the transporter. We determined that Ser-246 in the central intracellular loop plays a key role in the phosphorylation of Ynt1 and that the nitrogen permease reactivator 1 kinase (Npr1) is necessary for Ynt1 phosphorylation. Abolition of phosphorylation led Ynt1 to the vacuole by a pep12-dependent end4-independent pathway, which is also dependent on ubiquitinylation, whereas Ynt1 protein lacking ubiquitinylation sites does not follow this pathway. We found that, under nitrogen limitation, Ynt1 phosphorylation is essential for rapid induction of nitrate assimilation genes. Our results suggest that, under nitrogen limitation, phosphorylation prevents Ynt1 delivery from the secretion route to the vacuole, which, aided by reduced ubiquitinylation, accumulates Ynt1 at the plasma membrane. This mechanism could be part of the response that allows nitrate-assimilatory organisms to cope with nitrogen depletion.
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