ArticleBMC bioinformatics2008
AccPbFRET: an ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images.
Article in BMC bioinformatics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 67 citations in OpenAlex.
- Methods and Guidelines for Metabolism Studies: Applications to Cancer Research.International journal of molecular sciences · 2025Review
- Quantitative detection of amyloid fibrils using fluorescence resonance energy transfer between engineered yellow and cyan proteins.Protein science : a publication of the Protein Society · 2025Article
- PKCϵ-mediated phosphorylation of TRPC3 channel at S712 is essential for its inactivation during inflammatory signaling.Frontiers in immunology · 2025Article
- Measuring Molecular Interactions with Subcellular Resolution: Single-Cell FRET Using the Quantitative Three-Filterset (Intensity-Based) Approach.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity.Nature immunology · 2023Article
- cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8Immunity · 2023Article
- Pixel-by-pixel autofluorescence corrected FRET in fluorescence microscopy improves accuracy for samples with spatially varied autofluorescence to signal ratio.Scientific reports · 2023Article
- Low protease activity in B cell follicles promotes retention of intact antigens after immunization.Science (New York, N.Y.) · 2023Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Large scale, single-cell FRET-based glucose uptake measurements within heterogeneous populations.iScience · 2022Article
- Nanoscale Colocalization of NK Cell Activating and Inhibitory Receptors Controls Signal Integration.Frontiers in immunology · 2022Article
- A Protocol for FRET-Based Live-Cell Imaging in Microglia.STAR protocols · 2020Article
- PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways.Immunity · 2019Article
- Article
- Myristoylated rhinovirus VP4 protein activates TLR2-dependent proinflammatory gene expression.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells.Scientific reports · 2019Article
- In vivo topology converts competition for cell-matrix adhesion into directional migration.Nature communications · 2019Article
- Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells.Cell reports · 2018Article
- Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster.Journal of visualized experiments : JoVE · 2018Article
- Contactin-1/F3 Regulates Neuronal Migration and Morphogenesis Through Modulating RhoA Activity.Frontiers in molecular neuroscience · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe acceptor photobleaching fluorescence resonance energy transfer (FRET) method is widely used for monitoring molecular interactions in cells. This method of FRET, while among those with the simplest mathematics, is robust, self-controlled and independent of fluorophore amounts and ratios.
resultsAccPbFRET is a user-friendly, efficient ImageJ plugin which allows fully corrected, pixel-wise calculation and detailed, ROI (region of interest)-based analysis of FRET efficiencies in microscopic images. Furthermore, automatic registration and semi-automatic analysis of large image sets is provided, which are not available in any existing FRET evaluation software.
conclusionDespite of the widespread applicability of the acceptor photobleaching FRET technique, this is the first paper where all possible sources of major errors of the measurement and analysis are considered, and AccPbFRET is the only program which provides the complete suite of corrections--for registering image pairs, for unwanted photobleaching of the donor, for cross-talk of the acceptor and/or its photoproduct to the donor channel and for partial photobleaching of the acceptor. The program efficiently speeds up the analysis of large image sets even for novice users and is freely available.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.