ArticleNucleosides, nucleotides & nucleic acids2008
Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides.
Article in Nucleosides, nucleotides & nucleic acids, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 84 citations in OpenAlex.
- Sequencing and Optical Genome Mapping for the Adventurous Chemist.Chemical & biomedical imaging · 2024Review
- Real-time detection of human telomerase DNA synthesis by multiplexed single-molecule FRET.Biophysical journal · 2023Article
- Context-dependent DNA polymerization effects can masquerade as DNA modification signals.BMC genomics · 2022Article
- Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Enzymatic Cleavage of 3'-Esterified Nucleotides Enables a Long, Continuous DNA Synthesis.Scientific reports · 2020Article
- Article
- Engineering of Bioinspired, Size-Controllable, Self-Degradable Cancer-Targeting DNA Nanoflowers via the Incorporation of an Artificial Sandwich Base.Journal of the American Chemical Society · 2019Article
- Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing.Nature nanotechnology · 2017Article
- DNA sequencing using polymerase substrate-binding kinetics.Nature communications · 2015Article
- DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present.Frontiers in microbiology · 2014Review
- Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription.Journal of nanobiotechnology · 2013Article
- Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.BioTechniques · 2012Article
- Rapid incorporation kinetics and improved fidelity of a novel class of 3'-OH unblocked reversible terminators.Nucleic acids research · 2012Article
- Landscape of next-generation sequencing technologies.Analytical chemistry · 2011Review
- Fluorogenic DNA sequencing in PDMS microreactors.Nature methods · 2011Article
- Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide gamma-phosphate derivative.Nucleic acids research · 2011Article
- The properties and applications of single-molecule DNA sequencing.Genome biology · 2011Review
- Next generation sequencing for clinical diagnostics-principles and application to targeted resequencing for hypertrophic cardiomyopathy: a paper from the 2009 William Beaumont Hospital Symposium on Molecular Pathology.The Journal of molecular diagnostics : JMD · 2010Review
- Direct detection of DNA methylation during single-molecule, real-time sequencing.Nature methods · 2010Article
- Third-generation sequencing fireworks at Marco Island.Nature biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate the efficient synthesis of DNA with complete replacement of the four deoxyribonucleoside triphosphate (dNTP) substrates with nucleotides carrying fluorescent labels. A different, spectrally separable fluorescent dye suitable for single molecule fluorescence detection was conjugated to each of the four dNTPs via linkage to the terminal phosphate. Using these modified nucleotides, DNA synthesis by phi 29 DNA polymerase was observed to be processive for products thousands of bases in length, with labeled nucleotide affinities and DNA polymerization rates approaching unmodified dNTP levels. Results presented here show the compatibility of these nucleotides for single-molecule, real-time DNA sequencing applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.