ArticleProceedings of the National Academy of Sciences of the United States of America2008
Receptor density balances signal stimulation and attenuation in membrane-assembled complexes of bacterial chemotaxis signaling proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Bacterial chemoreceptor signaling complexes control kinase activity by stabilizing the catalytic domain of CheA.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Hydrogen exchange of chemoreceptors in functional complexes suggests protein stabilization mediates long-range allosteric coupling.The Journal of biological chemistry · 2019Article
- His-Tag-Mediated Dimerization of Chemoreceptors Leads to Assembly of Functional Nanoarrays.Biochemistry · 2017Article
- Signaling-Related Mobility Changes in Bacterial Chemotaxis Receptors Revealed by Solid-State NMR.The journal of physical chemistry. B · 2017Article
- A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues.Nature communications · 2016Article
- Preformed Soluble Chemoreceptor Trimers That Mimic Cellular Assembly States and Activate CheA Autophosphorylation.Biochemistry · 2015Article
- Hydrogen exchange differences between chemoreceptor signaling complexes localize to functionally important subdomains.Biochemistry · 2014Article
- ProBLM web server: protein and membrane placement and orientation package.Computational and mathematical methods in medicine · 2014Article
- Hydrogen exchange mass spectrometry of functional membrane-bound chemotaxis receptor complexes.Biochemistry · 2013Article
- Ligand affinity and kinase activity are independent of bacterial chemotaxis receptor concentration: insight into signaling mechanisms.Biochemistry · 2012Article
- Activated chemoreceptor arrays remain intact and hexagonally packed.Molecular microbiology · 2011Article
- Quantification of the brassinosteroid insensitive1 receptor in planta.Plant physiology · 2011Article
- Attractant binding induces distinct structural changes to the polar and lateral signaling clusters in Bacillus subtilis chemotaxis.The Journal of biological chemistry · 2011Article
- A dynamic-signaling-team model for chemotaxis receptors in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Spatial organization in bacterial chemotaxis.The EMBO journal · 2010Review
- Single-molecule atomic force microscopy reveals clustering of the yeast plasma-membrane sensor Wsc1.PloS one · 2010Article
- Article
- Bacterial chemoreceptors: providing enhanced features to two-component signaling.Current opinion in microbiology · 2010Review
- Disruption of chemoreceptor signalling arrays by high levels of CheW, the receptor-kinase coupling protein.Molecular microbiology · 2010Article
- The chemoreceptor dimer is the unit of conformational coupling and transmembrane signaling.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
All cells possess transmembrane signaling systems that function in the environment of the lipid bilayer. In the Escherichia coli chemotaxis pathway, the binding of attractants to a two-dimensional array of receptors and signaling proteins simultaneously inhibits an associated kinase and stimulates receptor methylation--a slower process that restores kinase activity. These two opposing effects lead to robust adaptation toward stimuli through a physical mechanism that is not understood. Here, we provide evidence of a counterbalancing influence exerted by receptor density on kinase stimulation and receptor methylation. Receptor signaling complexes were reconstituted over a range of defined surface concentrations by using a template-directed assembly method, and the kinase and receptor methylation activities were measured. Kinase activity and methylation rates were both found to vary significantly with surface concentration--yet in opposite ways: samples prepared at high surface densities stimulated kinase activity more effectively than low-density samples, whereas lower surface densities produced greater methylation rates than higher densities. FRET experiments demonstrated that the cooperative change in kinase activity coincided with a change in the arrangement of the membrane-associated receptor domains. The counterbalancing influence of density on receptor methylation and kinase stimulation leads naturally to a model for signal regulation that is compatible with the known logic of the E. coli pathway. Density-dependent mechanisms are likely to be general and may operate when two or more membrane-related processes are influenced differently by the two-dimensional concentration of pathway elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.