ArticleInfection and immunity2008
Identification and characterization of the Plasmodium yoelii PyP140/RON4 protein, an orthologue of Toxoplasma gondii RON4, whose cysteine-rich domain does not protect against lethal parasite challenge infection.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins.Communications biology · 2020Article
- Immunomic Identification of Malaria Antigens Associated With Protection in Mice.Molecular & cellular proteomics : MCP · 2019Article
- Size polymorphism and low sequence diversity in the locus encoding the Plasmodium vivax rhoptry neck protein 4 (PvRON4) in Colombian isolates.Malaria journal · 2016Article
- Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites.Scientific reports · 2016Article
- Identification and Characterization of the Rhoptry Neck Protein 2 in Babesia divergens and B. microti.Infection and immunity · 2016Article
- Crystal structure of PfRh5, an essential P. falciparum ligand for invasion of human erythrocytes.eLife · 2014Article
- Host cell invasion by apicomplexan parasites: the junction conundrum.PLoS pathogens · 2014Review
- Characterization of the interaction between Toxoplasma gondii rhoptry neck protein 4 and host cellular β-tubulin.Scientific reports · 2013Article
- Total and putative surface proteomics of malaria parasite salivary gland sporozoites.Molecular & cellular proteomics : MCP · 2013Article
- Article
- Peptide inhibitors of the malaria surface protein, apical membrane antigen 1: identification of key binding residues.Biopolymers · 2011Article
- The moving junction protein RON8 facilitates firm attachment and host cell invasion in Toxoplasma gondii.PLoS pathogens · 2011Article
- The RON2-AMA1 interaction is a critical step in moving junction-dependent invasion by apicomplexan parasites.PLoS pathogens · 2011Article
- Targeted disruption of py235ebp-1: invasion of erythrocytes by Plasmodium yoelii using an alternative Py235 erythrocyte binding protein.PLoS pathogens · 2011 · on this mapArticle
- Interaction between Plasmodium falciparum apical membrane antigen 1 and the rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites.The Journal of biological chemistry · 2010Article
- Structure of the Plasmodium falciparum circumsporozoite protein, a leading malaria vaccine candidate.The Journal of biological chemistry · 2009Article
- An inhibitory antibody blocks interactions between components of the malarial invasion machinery.PLoS pathogens · 2009Article
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12 authors.
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Abstract
Previously, we identified a Plasmodium yoelii YM 140-kDa merozoite protein, designated PyP140, which formed a complex with apical membrane antigen 1 (AMA1). Furthermore, we produced a nonprotective monoclonal antibody (MAb), 48F8, that immunoprecipitated metabolically labeled PyP140 and localized the protein to the merozoite's apical end and, less frequently, to the merozoite surface, as observed by immunofluorescence assay (IFA). Here, using MAb 48F8, we have identified the pyp140 gene by screening a P. yoelii lambda-Zap cDNA expression library. The pyp140 cDNA covers approximately 90% of the putative open reading frame (ORF) of PY02159 from the P. yoelii NL genome sequencing project. Analysis of the complete gene identified the presence of two introns. The ORF encodes a 102,407-Da protein with an amino-terminal signal sequence, a series of three unique types of repeats, and a cysteine-rich region. The binding site of MAb 48F8 was also identified. A BLAST search with the deduced amino acid sequence shows significant similarity with the Toxoplasma gondii RON4 protein and the Plasmodium falciparum RON4 protein, and the sequence is highly conserved in other Plasmodium species. We produced the cysteine-rich domain of PyP140/RON4 by using the Pichia pastoris expression system and characterized the recombinant protein biochemically and biophysically. BALB/c mice immunized with the protein formulated in oil-in-water adjuvants produced antibodies that recognize parasitized erythrocytes by IFA and native PyP140/RON4 by immunoblotting but failed to protect against a lethal P. yoelii YM infection. Our results show that PyP140/RON4 is located within the rhoptries or micronemes. It may associate in part with AMA1, but the conserved cysteine-rich domain does not appear to elicit inhibitory antibodies, a finding that is supported by the marked sequence conservation in this protein within Plasmodium spp., suggesting that it is not under immune pressure.
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