ArticleInfection and immunity2008
Prostaglandin E2 produced by Entamoeba histolytica binds to EP4 receptors and stimulates interleukin-8 production in human colonic cells.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Selected Protective Mechanisms of Human Milk Against Intestinal Protozoal Infections in Infants.Current issues in molecular biology · 2025Review
- Human Mucin-2-Producing Colonic Goblet-Like Cells Secrete the Chemokine CXCL8 by Activating Multiple Proinflammatory Pathways in Response to Entamoeba histolytica.The American journal of pathology · 2025Article
- Molecular regulation of NLRP3 inflammasome activation during parasitic infection.Bioscience reports · 2024Review
- Neutrophils versus Protozoan Parasites:Microorganisms · 2024Review
- The Gal/GalNac lectin as a possible acetylcholine receptor inFrontiers in cellular and infection microbiology · 2023Article
- Fatal attraction: intestinal amebiasis and COVID-19 as risk factors for colonic perforation.Journal of surgical case reports · 2021Article
- Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin EParasites & vectors · 2021Article
- Entamoeba histolytica Interaction with Enteropathogenic Escherichia coli Increases Parasite Virulence and Inflammation in Amebiasis.Infection and immunity · 2019Article
- Functional Characterization of an Interferon Gamma Receptor-Like Protein on Entamoeba histolytica.Infection and immunity · 2019Article
- Co-opting oxylipin signals in microbial disease.Cellular microbiology · 2019Review
- Learning from the research on amebiasis and gut microbiome: Is stimulation by gut flora essential for effective neutrophil mediated protection from external pathogens?Gut microbes · 2019Article
- Article
- Immune Response of Amebiasis and Immune Evasion by Entamoeba histolytica.Frontiers in immunology · 2016Review
- A whole-genome RNAi screen uncovers a novel role for human potassium channels in cell killing by the parasite Entamoeba histolytica.Scientific reports · 2015Article
- Experimental colitis in mice is attenuated by changes in the levels of endocannabinoid metabolites induced by selective inhibition of fatty acid amide hydrolase (FAAH).Journal of Crohn's & colitis · 2014Article
- Entamoeba histolytica-secreted cysteine proteases induce IL-8 production in human mast cells via a PAR2-independent mechanism.Parasite (Paris, France) · 2014Article
- NF-κB-dependent IL-8 induction by prostaglandin E(2) receptors EP(1) and EP(4).British journal of pharmacology · 2013Article
- The dynamic interdependence of amebiasis, innate immunity, and undernutrition.Seminars in immunopathology · 2012Review
- Leukotriene B(4) receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012Article
- Prostaglandin E2 produced by Entamoeba histolytica signals via EP4 receptor and alters claudin-4 to increase ion permeability of tight junctions.The American journal of pathology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Entamoeba histolytica pathogenesis in the colon occurs in a stepwise fashion. It begins with colonization of the mucin layer, which is followed by stimulation of a proinflammatory response that causes nonspecific tissue damage that may facilitate parasite invasion of the underlying colonic mucosa. Unfortunately, the parasite and/or host factors that stimulate a proinflammatory response in the gut are poorly understood. In this study, we found that live E. histolytica or secretory or proteins (SP) and soluble ameba components (SAP) can markedly increase interleukin-8 (IL-8) mRNA expression and protein production in colonic epithelial cells. The IL-8-stimulating molecule produced by live amebae was identified as prostaglandin E(2) (PGE(2)) as trophozoites treated with cyclooxygenase inhibitors inhibited the biosynthesis of PGE(2) and eliminated IL-8 production induced by live parasites or ameba components. Moreover, using specific prostaglandin EP2 and EP4 receptor agonists and antagonists, we found that PGE(2) binds exclusively through EP4 receptors in colonic epithelial cells to stimulate IL-8 production. Silencing of EP4 receptors with EP4 small interfering RNA completely eliminated SP- and SAP-induced IL-8 production. These studies identified bioactive PGE(2) as a one of the major virulence factors produced by E. histolytica that can stimulate the potent neutrophil chemokine and activator IL-8, which can trigger an acute host inflammatory response. Thus, the induction of IL-8 production in response to E. histolytica-derived PGE(2) may be a mechanism that explains the initiation and amplification of acute inflammation associated with intestinal amebiasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.