ArticleBMC cell biology2008
Interlobular and intralobular mammary stroma: genotype may not reflect phenotype.
Article in BMC cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Single-Cell Analysis in the Mouse and Human Mammary Gland.Advances in experimental medicine and biology · 2025Review
- Review
- Preclinical Models to Study Obesity and Breast Cancer in Females: Considerations, Caveats, and Tools.Journal of mammary gland biology and neoplasia · 2020Review
- Fibroblasts direct differentiation of human breast epithelial progenitors.Breast cancer research : BCR · 2020Article
- Upregulation of lipid metabolism genes in the breast prior to cancer diagnosis.NPJ breast cancer · 2020Article
- BRCA1 mutation influences progesterone response in human benign mammary organoids.Breast cancer research : BCR · 2019Article
- Persistent organic pollutants and obesity: are they potential mechanisms for breast cancer promotion?Endocrine-related cancer · 2015Review
- The role of lipolysis stimulated lipoprotein receptor in breast cancer and directing breast cancer cell behavior.PloS one · 2014Article
- Hornerin, an S100 family protein, is functional in breast cells and aberrantly expressed in breast cancer.BMC cancer · 2012Article
- Article
- Integrated morphodynamic signalling of the mammary gland.Nature reviews. Molecular cell biology · 2011Review
- Characterization of ductal and lobular breast carcinomas using novel prolactin receptor isoform specific antibodies.BMC cancer · 2010Article
- Lipid transfer proteins and protease inhibitors as key factors in the priming of barley responses to Fusarium head blight disease by a biocontrol strain of Pseudomonas fluorescens.Functional & integrative genomics · 2010Article
- The normal microenvironment directs mammary gland development.Journal of mammary gland biology and neoplasia · 2010Review
- Local regulation of human breast xenograft models.Journal of cellular physiology · 2010Article
- The normal breast microenvironment of premenopausal women differentially influences the behavior of breast cancer cells in vitro and in vivo.BMC medicine · 2010Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundThe normal growth and function of mammary epithelial cells depend on interactions with the supportive stroma. Alterations in this communication can lead to the progression or expansion of malignant growth. The human mammary gland contains two distinctive types of fibroblasts within the stroma. The epithelial cells are surrounded by loosely connected intralobular fibroblasts, which are subsequently surrounded by the more compacted interlobular fibroblasts. The different proximity of these fibroblasts to the epithelial cells suggests distinctive functions for these two subtypes. In this report, we compared the gene expression profiles between the two stromal subtypes.
methodsFresh normal breast tissue was collected from reduction mammoplasty patients and immediately placed into embedding medium and frozen on dry ice. Tissue sections were subjected to laser capture microscopy to isolate the interlobular from the intralobular fibroblasts. RNA was prepared and subjected to microarray analysis using the Affymetrix Human Genome U133 GeneChip. Data was analyzed using the Affy and Limma packages available from Bioconductor. Findings from the microarray analysis were validated by RT-PCR and immunohistochemistry.
resultsNo statistically significant difference was detected between the gene expression profiles of the interlobular and intralobular fibroblasts by microarray analysis and RT-PCR. However, for some of the genes tested, the protein expression patterns between the two subtypes of fibroblasts were significantly different.
conclusionThis study is the first to report the gene expression profiles of the two distinct fibroblast populations within the human mammary gland. While there was no significant difference in the gene expression profiles between the groups, there was an obvious difference in the expression pattern of several proteins tested. This report also highlights the importance of studying gene regulation at both the transcriptional and post-translational level.
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