ArticleGenes & development2008
Study of an RNA helicase implicates small RNA-noncoding RNA interactions in programmed DNA elimination in Tetrahymena.
Article in Genes & development, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
82 citing papers in PubMed.
- Transposon targeting non-coding RNA transcription targets G/C-rich tracts and is facilitated by an intrinsically disordered protein in Tetrahymena.Science China. Life sciences · 2026Article
- A histone methyltransferase-independent function of PRC2 controls small RNA dynamics during programmed DNA elimination in Paramecium.Nucleic acids research · 2025Article
- Soma-derived 30-nt small RNAs are coupled with chromosome breakage and precisely target nontransposon DNA against elimination inScience advances · 2025Article
- A specialized TFIIB is required for transcription of transposon-targeting noncoding RNAs.Nucleic acids research · 2025Article
- Functions and mechanisms of eukaryotic RNA-guided programmed DNA elimination.Biochemical Society transactions · 2025Review
- The PIWI-interacting protein Gtsf1 controls the selective degradation of small RNAs in Paramecium.Nucleic acids research · 2025Article
- A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination.eLife · 2024Article
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- Small RNA-directed DNA elimination: the molecular mechanism and its potential for genome editing.RNA biology · 2021Review
- A Polycomb repressive complex is required for RNAi-mediated heterochromatin formation and dynamic distribution of nuclear bodies.Nucleic acids research · 2021Article
- Article
- Roles of Noncoding RNAs in Ciliate Genome Architecture.Journal of molecular biology · 2020Review
- A distinct class of eukaryotic MT-A70 methyltransferases maintain symmetric DNA N6-adenine methylation at the ApT dinucleotides as an epigenetic mark associated with transcription.Nucleic acids research · 2019Article
- Environmentally induced plasticity of programmed DNA elimination boosts somatic variability inGenome research · 2019Article
- Functional Proteomics of Nuclear Proteins inGenes · 2019Review
- RNAi-dependentGenes & development · 2019Article
- RNA-mediated transgenerational inheritance in ciliates and plants.Chromosoma · 2018Review
- Whats, hows and whys of programmed DNA elimination inOpen biology · 2017Review
- A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.Nucleic acids research · 2017Article
- Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Tetrahymena eliminates micronuclear-limited sequences from the developing macronucleus during sexual reproduction. Homology between the sequences to be eliminated and approximately 28-nucleotide small RNAs (scnRNAs) associated with an Argonaute family protein Twi1p likely underlies this elimination process. However, the mechanism by which Twi1p-scnRNA complexes identify micronuclear-limited sequences is not well understood. We show that a Twi1p-associated putative RNA helicase Ema1p is required for the interaction between Twi1p and chromatin. This requirement explains the phenotypes of EMA1 KO strains, including loss of selective down-regulation of scnRNAs homologous to macronuclear-destined sequences, loss of H3K9 and K27 methylation in the developing new macronucleus, and failure to eliminate DNA. We further demonstrate that Twi1p interacts with noncoding transcripts derived from parental and developing macronuclei and this interaction is greatly reduced in the absence of Ema1p. We propose that Ema1p functions in DNA elimination by stimulating base-pairing interactions between scnRNAs and noncoding transcripts in both parental and developing new macronuclei.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.