ArticleEukaryotic cell2008
Identification of the putative protein phosphatase gene PTC1 as a virulence-related gene using a silkworm model of Candida albicans infection.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates.FEBS letters · 2026Review
- Bridging Manual and Computational Approaches: The Pseudophosphatase Scanner for Genome-Wide Fungal Pseudophosphatome Analysis.ACS omega · 2026Article
- Silkworm model of bacterial infection facilitates the identification of lysocin E, a potent, ultra-rapid bactericidal antibiotic.The Journal of antibiotics · 2024Review
- Alteration of β-glucan in the emerging fungal pathogen Candida auris leads to immune evasion and increased virulence.Medical microbiology and immunology · 2024Article
- Article
- Quantitative evaluation of Mycobacterium abscessus clinical isolate virulence using a silkworm infection model.PloS one · 2022Article
- Silkworm model forVirulence · 2021Article
- A small molecule produced by Lactobacillus species blocks Candida albicans filamentation by inhibiting a DYRK1-family kinase.Nature communications · 2021Article
- Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii.Scientific reports · 2021Article
- Article
- Pharmacokinetics of voriconazole and its alteration by Candida albicans infection in silkworms.The Journal of antibiotics · 2021Article
- Direct Visualization of Fungal Burden in Filamentous Fungus-Infected Silkworms.Journal of fungi (Basel, Switzerland) · 2021Article
- Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans.Nature communications · 2020Article
- A novel silkworm infection model with fluorescence imaging using transgenic Trichosporon asahii expressing eGFP.Scientific reports · 2020Article
- Identification and functional analysis of five genes that encode distinct isoforms of protein phosphatase 1 in Nilaparvata lugens.Scientific reports · 2020Article
- Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis.Microorganisms · 2020Review
- The phosphatase Ptc6 is involved in virulence and MAPK signalling in Fusarium oxysporum.Molecular plant pathology · 2020Article
- Identification and functional characterization of Candida albicans mannose-ethanolamine phosphotransferase (Mcd4p).Current genetics · 2019Article
- Silkworm as an experimental animal for research on fungal infections.Microbiology and immunology · 2019Review
- Silkworm, Bombyx mori, as an alternative model organism in toxicological research.Environmental science and pollution research international · 2018Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein phosphatases are critical for the regulation of many cellular processes. Null mutants of 21 putative protein phosphatases of Candida albicans were constructed by consecutive allele replacement using the URA3 and ARG4 marker genes. A simple silkworm model of C. albicans infection was used to screen the panel of mutants. Four null mutant (cmp1Delta, yvh1Delta, sit4Delta, and ptc1Delta) strains showed attenuated virulence in the silkworm model relative to that of control and parental strains. Three of the mutants, the cmp1Delta, yvh1Delta, and sit4Delta mutants, had previously been identified as affecting virulence in a conventional mouse model, indicating the validity of the silkworm model screen. Disruption of the putative protein phosphatase gene PTC1 of C. albicans, which has 52% identity to the Saccharomyces cerevisiae type 2C protein phosphatase PTC1, significantly reduced virulence in the silkworm model. The mutant was also avirulent in a mouse model of disseminated candidiasis. Reintroducing either of the C. albicans PTC1 alleles into the disruptant strain, using a cassette containing either allele under the control of a constitutive ACT1 promoter, restored virulence in both infection models. Characterization of ptc1Delta revealed other phenotypic traits, including reduced hyphal growth in vitro and in vivo, and reduced extracellular proteolytic activity. We conclude that PTC1 may contribute to pathogenicity in C. albicans.
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