ArticleApplied and environmental microbiology2008
Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Foodborne pathogenic bacteria in wild European hedgehogs (Erinaceus europaeus).Acta veterinaria Scandinavica · 2024Article
- EnteropathogenicPathogens (Basel, Switzerland) · 2024Article
- A novel approach for simultaneous detection of the most common food-borne pathogens by multiplex qPCR.Biomolecules & biomedicine · 2023Article
- Prevalence, Genetic Homogeneity, and Antibiotic Resistance of PathogenicAntibiotics (Basel, Switzerland) · 2023Article
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Establishment of a Real-Time Recombinase Polymerase Amplification for Rapid Detection of PathogenicPathogens (Basel, Switzerland) · 2023Article
- Ultra-Sensitive and Rapid Detection of PathogenicFoods (Basel, Switzerland) · 2022Article
- Characterisation ofJournal of veterinary research · 2022Article
- Yersiniosis in New Zealand.Pathogens (Basel, Switzerland) · 2021Review
- Performance of the QIAstat-Dx Gastrointestinal Panel for Diagnosing Infectious Gastroenteritis.Journal of clinical microbiology · 2020Article
- Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control.PloS one · 2020Article
- Review
- Prevalence and genomic characteristics of zoonotic gastro-intestinal pathogens and ESBL/pAmpC producingInfection ecology & epidemiology · 2019Article
- Distribution of enteropathogenic Yersinia spp. and Salmonella spp. in the Swedish wild boar population, and assessment of risk factors that may affect their prevalence.Acta veterinaria Scandinavica · 2018Article
- Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR.Polish journal of microbiology · 2018Article
- Prevalence of human pathogenic Yersinia enterocolitica in Swedish pig farms.Acta veterinaria Scandinavica · 2018Article
- Detection Methodologies for Pathogen and Toxins: A Review.Sensors (Basel, Switzerland) · 2017Review
- Raw Meat-Based Diets in Dogs and Cats.Veterinary sciences · 2017Article
- Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in the Basque Country, northern Spain.Acta veterinaria Scandinavica · 2016Article
- Multispecies Epidemiologic Surveillance Study after an Outbreak of Yersiniosis at an African Green Monkey Research Facility.Comparative medicine · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current methods for the detection of pathogenic Yersinia enterocolitica bacteria in food are time consuming and inefficient. Therefore, we have developed and evaluated in-house a TaqMan probe-based real-time PCR method for the detection of this pathogen. The complete method comprises overnight enrichment, DNA extraction, and real-time PCR amplification. Also included in the method is an internal amplification control. The selected primer-probe set was designed to use a 163-bp amplicon from the chromosomally located gene ail (attachment and invasion locus). The selectivity of the PCR method was tested with a diverse range (n = 152) of related and unrelated strains, and no false-negative or false-positive PCR results were obtained. The sensitivity of the PCR amplification was 85 fg purified genomic DNA, equivalent to 10 cells per PCR tube. Following the enrichment of 10 g of various food samples (milk, minced beef, cold-smoked sausage, fish, and carrots), the sensitivity ranged from 0.5 to 55 CFU Y. enterocolitica. Good precision, robustness, and efficiency of the PCR amplification were also established. In addition, the method was tested on naturally contaminated food; in all, 18 out of 125 samples were positive for the ail gene. Since no conventional culture method could be used as a reference method, the PCR products amplified from these samples were positively verified by using conventional PCR and sequencing of the amplicons. A rapid and specific real-time PCR method for the detection of pathogenic Y. enterocolitica bacteria in food, as presented here, provides a superior alternative to the currently available detection methods and makes it possible to identify the foods at risk for Y. enterocolitica contamination.
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