ArticleApplied and environmental microbiology2008
Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 136 citations in OpenAlex.
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- Beer yeast breeding in the era of innovation: advances and applications for modern brewing.Biotechnology letters · 2025Review
- Factors Affecting D-Lactic Acid Production by FlocculantMicroorganisms · 2025Article
- Functional analysis of Saccharomyces cerevisiae FLO genes through optogenetic control.FEMS yeast research · 2025Article
- Effects of regional location on the genotype and phenotype of historical Irish brewing yeast.Frontiers in microbiology · 2025Article
- Fungal biofilm formation and its regulatory mechanism.Heliyon · 2024Review
- Strain and temperature dependent aggregation ofCell surface (Amsterdam, Netherlands) · 2023Article
- Heterologous Expression ofJournal of fungi (Basel, Switzerland) · 2022Article
- Drug-dependent growth curve reshaping reveals mechanisms of antifungal resistance in Saccharomyces cerevisiae.Communications biology · 2022Article
- EngineeredBioengineered · 2020Review
- Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family.FEMS yeast research · 2020Article
- Identification of flocculant wine yeast strains with improved filtration-related phenotypes through application of high-throughput sedimentation rate assays.Scientific reports · 2020Article
- Flocculation and Expression ofFood technology and biotechnology · 2019Article
- Lager-brewing yeasts in the era of modern genetics.FEMS yeast research · 2019Review
- Cell Aggregation and Aerobic Respiration Are Important forApplied and environmental microbiology · 2019Article
- Modifying Saccharomyces cerevisiae Adhesion Properties Regulates Yeast Ecosystem Dynamics.mSphere · 2018Article
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many industrial fermentation processes, the Saccharomyces cerevisiae yeast should ideally meet two partially conflicting demands. During fermentation, a high suspended yeast count is required to maintain a satisfactory rate of fermentation, while at completion, efficient settling is desired to enhance product clarification and recovery. In most fermentation industries, currently used starter cultures do not satisfy this ideal, probably because nonflocculent yeast strains were selected to avoid fermentation problems. In this paper, we assess molecular strategies to optimize the flocculation behavior of S. cerevisiae. For this purpose, the chromosomal copies of three dominant flocculation genes, FLO1, FLO5, and FLO11, of the haploid nonflocculent, noninvasive, and non-flor-forming S. cerevisiae FY23 strain were placed under the transcriptional control of the promoters of the ADH2 and HSP30 genes. All six promoter-gene combinations resulted in specific flocculation behaviors in terms of timing and intensity. The strategy resulted in stable expression patterns providing a platform for the direct comparison and assessment of the specific impact of the expression of individual dominant FLO genes with regard to cell wall characteristics, such as hydrophobicity, biofilm formation, and substrate adhesion properties. The data also clearly demonstrate that the flocculation behavior of yeast strains can be tightly controlled and fine-tuned to satisfy specific industrial requirements.
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