ArticleApplied and environmental microbiology2008
Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an enzyme that is not essential for cyanide assimilation.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Nitrogen Removal Characteristics and Application by a Novel Cold-Resistant Bacterium.Microorganisms · 2026Article
- Characterization of a Periplasmic D-Malate:CytochromeInternational journal of molecular sciences · 2025Article
- Entrapment of Cyanase fromPolymers · 2024Article
- Genomic Insights into Cyanide Biodegradation in theInternational journal of molecular sciences · 2024Article
- Heterologous Expression of Cyanobacterial Cyanase Gene (Biology · 2022Article
- Horizontal gene transfer provides insights into the deep evolutionary history and biology ofFood and waterborne parasitology · 2022Article
- Alternative Pathway for 3-Cyanoalanine Assimilation in Pseudomonas pseudoalcaligenes CECT5344 under Noncyanotrophic Conditions.Microbiology spectrum · 2021Article
- Evidence for Selection in the Abundant Accessory Gene Content of a Prokaryote Pangenome.Molecular biology and evolution · 2021Article
- Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation.Scientific reports · 2021Article
- Use of Newly Designed Primers for Quantification of Complete Ammonia-Oxidizing (Comammox) Bacterial Clades and Strict Nitrite Oxidizers in the GenusApplied and environmental microbiology · 2020Article
- Bacterial non-specific nucleases of the phospholipase D superfamily and their biotechnological potential.Applied microbiology and biotechnology · 2020Review
- Role of the Dihydrodipicolinate Synthase DapA1 on Iron Homeostasis During Cyanide Assimilation by the Alkaliphilic BacteriumFrontiers in microbiology · 2020Article
- Interaction of cyanate uptake by rice seedlings with nitrate assimilation: gene expression analysis.Environmental science and pollution research international · 2019Article
- Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading BacteriumInternational journal of molecular sciences · 2019Article
- A review on remediation of cyanide containing industrial wastes using biological systems with special reference to enzymatic degradation.World journal of microbiology & biotechnology · 2019Review
- Complete nitrification: insights into the ecophysiology of comammox Nitrospira.Applied microbiology and biotechnology · 2019Review
- Cyanase-independent utilization of cyanate as a nitrogen source in ascomycete yeasts.World journal of microbiology & biotechnology · 2018Article
- Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists.Applied and environmental microbiology · 2018Article
- Assimilation of cyanide and cyano-derivatives by Pseudomonas pseudoalcaligenes CECT5344: from omic approaches to biotechnological applications.FEMS microbiology letters · 2018Article
- A Cyanide-Induced 3-Cyanoalanine Nitrilase in the Cyanide-Assimilating Bacterium Pseudomonas pseudoalcaligenes Strain CECT 5344.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detected in cell extracts from cells grown with ammonium, even in the presence of cyanate. Nevertheless, a low level of cyanase activity was detected in nitrogen-starved cells. The cyn gene cluster of P. pseudoalcaligenes CECT5344 was cloned and analyzed. The cynA, cynB, and cynD genes encode an ABC-type transporter, the cynS gene codes for the cyanase, and the cynF gene encodes a novel sigma(54)-dependent transcriptional regulator which is not present in other bacterial cyn gene clusters. The CynS protein was expressed in Escherichia coli and purified by following a simple and rapid protocol. The P. pseudoalcaligenes cyanase showed an optimal pH of 8.5 degrees C and a temperature of 65 degrees C. An insertion mutation was generated in the cynS gene. The resulting mutant was unable to use cyanate as the sole nitrogen source but showed the same resistance to cyanate as the wild-type strain. These results, in conjunction with the induction pattern of the enzymatic activity, suggest that the enzyme has an assimilatory function. Although the induction of cyanase activity in cyanide-degrading cells suggests that some cyanate may be generated from cyanide, the cynS mutant was not affected in its ability to degrade cyanide, which unambiguously indicates that cyanate is not a central metabolite in cyanide assimilation.
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