ArticleJournal of bacteriology2008
Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
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- First Molecular Characterization and Antibiogram of Bacteria Isolated From Dairy Farm Wastewater in Bangladesh.Veterinary medicine international · 2025Article
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- Unveiling antimicrobial resistance in Chilean fertilized soils: a One Health perspective on environmental AMR surveillance.Frontiers in microbiology · 2023Article
- Characterization of Adherent-InvasiveInternational journal of molecular sciences · 2022Article
- Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR.Microbiology spectrum · 2022Article
- Iron Transport and Metabolism in Escherichia, Shigella, and Salmonella.EcoSal Plus · 2021Article
- Candidatus Mycoplasma haemohominis in Human, Japan.Emerging infectious diseases · 2020Article
- Virulence behavior of uropathogenic Escherichia coli strains in the host model Caenorhabditis elegans.MicrobiologyOpen · 2019Article
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- Enhanced Biofilm Formation and Membrane Vesicle Release byFrontiers in microbiology · 2018Article
- Molecular Mechanism and Genetic Determinants of Buprofezin Degradation.Applied and environmental microbiology · 2017Article
- Genome-Based Comparison of Cyclic Di-GMP Signaling in Pathogenic and Commensal Escherichia coli Strains.Journal of bacteriology · 2016Article
- Characterization of epidemic IncI1-Iγ plasmids harboring ambler class A and C genes in Escherichia coli and Salmonella enterica from animals and humans.Antimicrobial agents and chemotherapy · 2015Article
- Whole-genome sequencing targets drug-resistant bacterial infections.Human genomics · 2015Review
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7 authors.
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Abstract
The increasing occurrence of multidrug-resistant pathogens of clinical and agricultural importance is a global public health concern. While antimicrobial use in human and veterinary medicine is known to contribute to the dissemination of antimicrobial resistance, the impact of microbial communities and mobile resistance genes from the environment in this process is not well understood. Isolated from an industrially polluted aquatic environment, Escherichia coli SMS-3-5 is resistant to a record number of antimicrobial compounds from all major classes, including two front-line fluoroquinolones (ciprofloxacin and moxifloxacin), and in many cases at record-high concentrations. To gain insights into antimicrobial resistance in environmental bacterial populations, the genome of E. coli SMS-3-5 was sequenced and compared to the genome sequences of other E. coli strains. In addition, selected genetic loci from E. coli SMS-3-5 predicted to be involved in antimicrobial resistance were phenotypically characterized. Using recombinant vector clones from shotgun sequencing libraries, resistance to tetracycline, streptomycin, and sulfonamide/trimethoprim was assigned to a single mosaic region on a 130-kb plasmid (pSMS35_130). The remaining plasmid backbone showed similarity to virulence plasmids from avian-pathogenic E. coli (APEC) strains. Individual resistance gene cassettes from pSMS35_130 are conserved among resistant bacterial isolates from multiple phylogenetic and geographic sources. Resistance to quinolones was assigned to several chromosomal loci, mostly encoding transport systems that are also present in susceptible E. coli isolates. Antimicrobial resistance in E. coli SMS-3-5 is therefore dependent both on determinants acquired from a mobile gene pool that is likely available to clinical and agricultural pathogens, as well, and on specifically adapted multidrug efflux systems. The association of antimicrobial resistance with APEC virulence genes on pSMS35_130 highlights the risk of promoting the spread of virulence through the extensive use of antibiotics.
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