ArticleThe Journal of biological chemistry2008
Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Chemical biology research in RIKEN NPDepo aimed at agricultural applications.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- The Impact of Heterologous Regulatory Genes from Lipodepsipeptide Biosynthetic Gene Clusters on the Production of Teicoplanin and A40926.Antibiotics (Basel, Switzerland) · 2024Article
- Studies on Streptomyces sp. SN-593: reveromycin biosynthesis, β-carboline biomediator activating LuxR family regulator, and construction of terpenoid biosynthetic platform.The Journal of antibiotics · 2022Review
- Florigen and its homologs of FT/CETS/PEBP/RKIP/YbhB family may be the enzymes of small molecule metabolism: review of the evidence.BMC plant biology · 2022Review
- Identification and Predictions Regarding the Biosynthesis Pathway of Polyene Macrolides Produced by Streptomyces roseoflavus Men-myco-93-63.Applied and environmental microbiology · 2021Article
- Early Steps in the Biosynthetic Pathway of Rishirilide B.Molecules (Basel, Switzerland) · 2020Article
- The biosynthetic pathway to tetromadurin (SF2487/A80577), a polyether tetronate antibiotic.PloS one · 2020Article
- Computational identification of co-evolving multi-gene modules in microbial biosynthetic gene clusters.Communications biology · 2019Article
- Characterization of the biosynthetic gene cluster of the polyene macrolide antibiotic reedsmycins from a marine-derived Streptomyces strain.Microbial cell factories · 2018Article
- SeMPI: a genome-based secondary metabolite prediction and identification web server.Nucleic acids research · 2017Article
- Biosynthesis, regulation, and engineering of a linear polyketide tautomycetin: a novel immunosuppressant in Streptomyces sp. CK4412.Journal of industrial microbiology & biotechnology · 2017Review
- Oxidative Cyclization in Natural Product Biosynthesis.Chemical reviews · 2017Review
- Chemical and biological studies of reveromycin A.The Journal of antibiotics · 2016Review
- Eco-friendly approach using marine actinobacteria and its compounds to control ticks and mosquitoes.Parasitology research · 2013Article
- Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation.Nature chemical biology · 2011Article
- Characterization of the biosynthesis gene cluster for the pyrrole polyether antibiotic calcimycin (A23187) in Streptomyces chartreusis NRRL 3882.Antimicrobial agents and chemotherapy · 2011Article
- SHP2 is a target of the immunosuppressant tautomycetin.Chemistry & biology · 2011Article
- Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units.The Journal of biological chemistry · 2010Article
- Functional characterization of TtnD and TtnF, unveiling new insights into tautomycetin biosynthesis.Journal of the American Chemical Society · 2010Article
- Functional expression of SCO7832 stimulates tautomycetin production via pathway-specific regulatory gene overexpression in Streptomyces sp. CK4412.Journal of industrial microbiology & biotechnology · 2009Article
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5 authors.
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Abstract
Tautomycin (TTM) is a highly potent and specific protein phosphatase inhibitor isolated from Streptomyces spiroverticillatus. The biological activity of TTM makes it an important lead for drug discovery, whereas its spiroketal-containing polyketide chain and rare dialkylmaleic anhydride moiety draw attention to novel biosynthetic chemistries responsible for its production. To elucidate the biosynthetic machinery associated with these novel molecular features, the ttm biosynthetic gene cluster from S. spiroverticillatus was isolated and characterized, and its involvement in TTM biosynthesis was confirmed by gene inactivation and complementation experiments. The ttm cluster was localized to a 86-kb DNA region, consisting of 20 open reading frames that encode three modular type I polyketide synthases (TtmHIJ), one type II thioesterase (TtmT), five proteins for methoxymalonyl-S-acyl carrier protein biosynthesis (Ttm-ABCDE), eight proteins for dialkylmaleic anhydride biosynthesis and regulation (TtmKLMNOPRS), as well as two additional regulatory proteins (TtmF and TtmQ) and one tailoring enzyme (TtmG). A model for TTM biosynthesis is proposed based on functional assignments from sequence analysis, which agrees well with previous feeding experiments, and has been further supported by in vivo gene inactivation experiments. These findings set the stage to fully investigate TTM biosynthesis and to biosynthetically engineer new TTM analogs.
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