ArticleInternational journal for parasitology2009
The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti.
Article in International journal for parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- REVIEW: THE ORIGIN OF DIGENETIC TREMATODES: DID THE STORY BEGIN WITH THE BLOOD FLUKES?The Journal of parasitology · 2025Review
- Schistosomiasis: cercarial finding and recognizing of human hosts as a prerequisite of invasion.Clinical microbiology reviews · 2025Review
- Schistosoma japonicum cathepsin B2 (SjCB2) facilitates parasite invasion through the skin.PLoS neglected tropical diseases · 2020Article
- Nitric oxide debilitates the neuropathogenic schistosome Trichobilharzia regenti in mice, partly by inhibiting its vital peptidases.Parasites & vectors · 2020Article
- Molecular evidence for distinct modes of nutrient acquisition between visceral and neurotropic schistosomes of birds.Scientific reports · 2019Article
- Cysteine proteases during larval migration and development of helminths in their final host.PLoS neglected tropical diseases · 2018Review
- Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp.Parasites & vectors · 2018Article
- Lessons from the genomes and transcriptomes of filarial nematodes.Molecular and biochemical parasitology · 2017Review
- Article
- Nitric oxide and cytokine production by glial cells exposed in vitro to neuropathogenic schistosome Trichobilharzia regenti.Parasites & vectors · 2016Article
- Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host.PLoS neglected tropical diseases · 2016Article
- Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti.Parasites & vectors · 2015Article
- Avian schistosomes and outbreaks of cercarial dermatitis.Clinical microbiology reviews · 2015Review
- Expression profile of the Schistosoma japonicum degradome reveals differential protease expression patterns and potential anti-schistosomal intervention targets.PLoS computational biology · 2014Article
- Cercarial dermatitis, a neglected allergic disease.Clinical reviews in allergy & immunology · 2013Review
- Investigation of the proteolytic functions of an expanded cercarial elastase gene family in Schistosoma mansoni.PLoS neglected tropical diseases · 2012Article
- Pathogenicity of Trichobilharzia spp. for Vertebrates.Journal of parasitology research · 2012Article
- Proteomic analysis of human skin treated with larval schistosome peptidases reveals distinct invasion strategies among species of blood flukes.PLoS neglected tropical diseases · 2011Article
- Secretory glands in cercaria of the neuropathogenic schistosome Trichobilharzia regenti - ultrastructural characterization, 3-D modelling, volume and pH estimations.Parasites & vectors · 2011Article
- Molecular cloning and characterization of a cathepsin B from Angiostrongylus cantonensis.Parasitology research · 2011Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
A transcriptional product of a gene encoding cathepsin B-like peptidase in the bird schistosome Trichobilharzia regenti was identified and cloned. The enzyme was named TrCB2 due to its 77% sequence similarity to cathepsin B2 from the important human parasite Schistosoma mansoni. The zymogen was expressed in the methylotropic yeast Pichia pastoris; procathepsin B2 underwent self-processing in yeast media. The peptidolytic activity of the recombinant enzyme was characterised using synthetic fluorogenic peptide substrates at optimal pH 6.0. Functional studies using different specific inhibitors proved the typical cathepsin B-like nature of the enzyme. The S(2) subsite specificity profile of recombinant TrCB2 was obtained. Using monospecific antibodies against the recombinant enzyme, the presence of cathepsin B2 was confirmed in extracts from cercariae (infective stage) and schistosomula (early post-cercarial stage) of T. regenti on Western blots. Also, cross-reactivity was observed between T. regenti and S. mansoni cathepsins B2 in extracts of cercariae, schistosomula or adults. In T. regenti, the antisera localised the enzyme to post-acetabular penetration glands of cercariae implying an important role in the penetration of host skin. The ability of recombinant TrCB2 to degrade skin, serum and nervous tissue proteins was evident. Elastinolytic activity suggests that the enzyme might functionally substitute the histolytic role of the serine class elastase known from S. mansoni and Schistosoma haematobium but not found in Schistosoma japonicum or in bird schistosomes.
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