ArticleJournal of structural biology2008
Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection.
Article in Journal of structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM.Journal of structural biology · 2025Article
- Article
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- MagIC beads for scarce macromolecules.eLife · 2025Article
- Open architecture of archaea MCM and dsDNA complexes resolved using monodispersed streptavidin affinity CryoEM.Nature communications · 2024Article
- Self-assembled superstructure alleviates air-water interface effect in cryo-EM.Nature communications · 2024Article
- A 3D-printed flow-cell for on-grid purification of electron microscopy samples directly from lysate.Journal of structural biology · 2023Article
- Article
- Developing Graphene Grids for Cryoelectron Microscopy.Frontiers in molecular biosciences · 2022Review
- Recent Technical Advances in Sample Preparation for Single-Particle Cryo-EM.Frontiers in molecular biosciences · 2022Review
- A cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI.Nature communications · 2021Article
- From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cryo-EM sample preparation method for extremely low concentration liposomes.Ultramicroscopy · 2020Article
- Cryo-EM studies of NAIP-NLRC4 inflammasomes.Methods in enzymology · 2019Article
- A simple and robust procedure for preparing graphene-oxide cryo-EM grids.Journal of structural biology · 2018Article
- Biological Applications at the Cutting Edge of Cryo-Electron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Review
- PROTEINS, INTERFACES, AND CRYO-EM GRIDS.Current opinion in colloid & interface science · 2018Article
- Monolayer-crystal streptavidin support films provide an internal standard of cryo-EM image quality.Journal of structural biology · 2017Article
- Cryo-EM: beyond the microscope.Current opinion in structural biology · 2017Review
- Opinion: hazards faced by macromolecules when confined to thin aqueous films.Biophysics reports · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
For cryo-EM structural studies, we seek to image membrane proteins as single particles embedded in proteoliposomes. One technical difficulty has been the low density of liposomes that can be trapped in the approximately 100nm ice layer that spans holes in the perforated carbon support film of EM grids. Inspired by the use of two-dimensional (2D) streptavidin crystals as an affinity surface for biotinylated DNA (Crucifix et al., 2004), we propose to use the crystals to tether liposomes doped with biotinylated lipids. The 2D crystal image also serves as a calibration of the image formation process, providing an absolute conversion from electrostatic potentials in the specimen to the EM image intensity, and serving as a quality control of acquired cryo-EM images. We were able to grow streptavidin crystals covering more than 90% of the holes in an EM grid, and which remained stable even under negative stain. The liposome density in the resulting cryo-EM sample was uniform and high due to the high-affinity binding of biotin to streptavidin. Using computational methods, the 2D crystal background can be removed from images without noticeable effect on image properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.