ArticleMolecular plant pathology2008
Mining the secretome of the root-knot nematode Meloidogyne chitwoodi for candidate parasitism genes.
Article in Molecular plant pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 77 citations in OpenAlex.
- Genome reannotation and gland-specific transcriptome analysis identify new effector candidates in Meloidogyne chitwoodi.PLoS pathogens · 2025Article
- Article
- Transcriptome Analysis ofPlants (Basel, Switzerland) · 2024Article
- Exploiting Plant-Phytonematode Interactions to Upgrade Safe and Effective Nematode Control.Life (Basel, Switzerland) · 2022Review
- Understanding Molecular Plant-Nematode Interactions to Develop Alternative Approaches for Nematode Control.Plants (Basel, Switzerland) · 2022Review
- Oxylipins are implicated as communication signals in tomato-root-knot nematode (Meloidogyne javanica) interaction.Scientific reports · 2021Article
- Effectors of Root-Knot Nematodes: An Arsenal for Successful Parasitism.Frontiers in plant science · 2021Review
- Transcriptome Analyses of Pre-parasitic and ParasiticJournal of nematology · 2021Article
- A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism.Molecular plant pathology · 2019Article
- Knockdown of a mucin-like gene in Meloidogyne incognita (Nematoda) decreases attachment of endospores of Pasteuria penetrans to the infective juveniles and reduces nematode fecundity.Molecular plant pathology · 2018Article
- Signal Transduction in Plant⁻Nematode Interactions.International journal of molecular sciences · 2018Review
- Smart Parasitic Nematodes Use Multifaceted Strategies to Parasitize Plants.Frontiers in plant science · 2017Review
- Identification and functional analysis of secreted effectors from phytoparasitic nematodes.BMC microbiology · 2016Review
- Msp40 effector of root-knot nematode manipulates plant immunity to facilitate parasitism.Scientific reports · 2016Article
- Cellular Signaling Pathways and Posttranslational Modifications Mediated by Nematode Effector Proteins.Plant physiology · 2015Review
- Mining novel effector proteins from the esophageal gland cells of Meloidogyne incognita.Molecular plant-microbe interactions : MPMI · 2014Article
- Transcriptomic analysis of the rice white tip nematode, Aphelenchoides besseyi (Nematoda: Aphelenchoididae).PloS one · 2014Article
- Harnessing the helminth secretome for therapeutic immunomodulators.BioMed research international · 2014Review
- Identification of candidate effector genes in the transcriptome of the rice root knot nematode Meloidogyne graminicola.Molecular plant pathology · 2013Article
- Exploring the host parasitism of the migratory plant-parasitic nematode Ditylenchus destuctor by expressed sequence tags analysis.PloS one · 2013Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Parasite proteins secreted at the interface of nematode and host are believed to play an essential role in parasitism. Here, we present an efficient pipeline of bio-informatic algorithms and laboratory experiments to identify candidate parasitism genes within nematode secretomes, i.e. the repertoire of secreted proteins in an organism. We performed our approach on 12 218 expressed sequence tags (ESTs) originating from three life stages of the plant parasitic nematode Meloidogyne chitwoodi--a molecularly unexplored root-knot nematode species. The ESTs from M. chitwoodi were assembled into 5880 contigs and open reading frames translated from the consensus sequences were searched for features of putative signal peptides for protein secretion and trans-membrane regions, resulting in the identification of 398 secretome members. The products of parasitism genes are secreted by a range of organs, including the oesophageal, amphidial and rectal glands, the intestine, and the hypodermis. To localize the site of expression in M. chitwoodi, we subjected the most abundant secretome members to in situ hybridization microscopy. We found hybridization of one tag in the dorsal oesophageal gland, seven in the two subventral oesophageal glands, two in the intestine and one tag hybridized to the tail tip in the proximity of the phasmids. Four sequences showed similarity to putative parasitism genes from other nematode species, whereas seven represented pioneering sequences. Our approach presents an efficient method to identify candidate parasitism genes, which does not require sophisticated cDNA isolation and selection protocols, and can therefore be used as a powerful starting point for the molecular investigation of parasites.
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