ArticleMolecular plant pathology2008
Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips.
Article in Molecular plant pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 190 citations in OpenAlex.
- A model-guided alternating high-temperature thermotherapy achieves complete eradication of sugarcane mosaic virus and preserves physiological integrity inFrontiers in plant science · 2026Article
- Virus Elimination by Thermotherapy Combined with Meristem Culture.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cryopreservation of Rubus viruses in raspberry shoot tips via droplet-vitrification: assessment of viral preservation, localization, and post-thaw transmission capacity.Plant methods · 2025Article
- Managing Plant Viruses in Tissue-Cultured Apple and Grapevine: Strategies for Detection and Eradication.The plant pathology journal · 2025Review
- Review
- Optimization of in vitro propagation and virus eradication using meristem culture and thermotherapy in two geranium species Pelargonium X hortorum ('Zonal') and Pelargonium × domesticum ('Regal').BMC plant biology · 2025Article
- Assessing the efficacy of thermotherapy combined with chemotherapy or cryotherapy for the eradication of grapevine leafroll-associated virus 3.Frontiers in plant science · 2025Article
- Efficacy of Plant Tissue Culture Techniques for Eliminating Black Mulberry Idaeovirus (BMIV) from Infected Black Mulberry (Plants (Basel, Switzerland) · 2024Article
- The Elimination of Viroids through In Vitro Thermotherapy and a Meristem Tip Culture from a New Limonime Hybrid (Biotech (Basel (Switzerland)) · 2024Article
- Integrated Management of Pathogens and Microbes inPlants (Basel, Switzerland) · 2024Review
- An Overview of Mycoviral Curing Strategies Used in Evaluating Fungal Host Fitness.Molecular biotechnology · 2023Review
- Seed transmission of raspberry bushy dwarf virus is blocked in Nicotiana benthamiana plants by preventing virus entry into the embryo from the infected embryo sac and endosperm.Archives of virology · 2023Article
- Review
- Elimination of Eight Viruses and Two Viroids from Preclonal Candidates of Six Grapevine Varieties (Plants (Basel, Switzerland) · 2022Article
- Thermotherapy Followed by Shoot Tip Cryotherapy Eradicates Latent Viruses and Apple Hammerhead Viroid from In Vitro Apple Rootstocks.Plants (Basel, Switzerland) · 2022Article
- Eradication ofFrontiers in plant science · 2022Article
- Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions.Plants (Basel, Switzerland) · 2021Review
- Osmo- and cryotherapy of sugarcane (Virusdisease · 2020Article
- Efficient elimination ofSouth African journal of botany : official journal of the South African Association of Botanists = Suid-Afrikaanse tydskrif vir plantkunde : amptelike tydskrif van die Suid-Afrikaanse Genootskap van Plantkundiges · 2020Article
- In vitro tissue culture of apple and other Malus species: recent advances and applications.Planta · 2019Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accumulation of viruses in vegetatively propagated plants causes heavy yield losses. Therefore, supply of virus-free planting materials is pivotal to sustainable crop production. In previous studies, Raspberry bushy dwarf virus (RBDV) was difficult to eradicate from raspberry (Rubus idaeus) using the conventional means of meristem tip culture. As shown in the present study, it was probably because this pollen-transmitted virus efficiently invades leaf primordia and all meristematic tissues except the least differentiated cells of the apical dome. Subjecting plants to thermotherapy prior to meristem tip culture heavily reduced viral RNA2, RNA3 and the coat protein in the shoot tips, but no virus-free plants were obtained. Therefore, a novel method including thermotherapy followed by cryotherapy was developed for efficient virus eradication. Heat treatment caused subcellular alterations such as enlargement of vacuoles in the more developed, virus-infected cells, which were largely eliminated following subsequent cryotherapy. Using this protocol, 20-36% of the treated shoot tips survived, 30-40% regenerated and up to 35% of the regenerated plants were virus-free, as tested by ELISA and reverse transcription loop-mediated isothermal amplification. Novel cellular and molecular insights into RBDV-host interactions and the factors influencing virus eradication were obtained, including invasion of shoot tips and meristematic tissues by RBDV, enhanced viral RNA degradation and increased sensitivity to freezing caused by thermotherapy, and subcellular changes and subsequent death of cells caused by cryotherapy. This novel procedure should be helpful with many virus-host combinations in which virus eradication by conventional means has proven difficult.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.