ArticleProteomics2008
Flow cytometry-assisted purification and proteomic analysis of the corticotropes dense-core secretory granules.
Article in Proteomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Organelle Sorting and Proteomic Analysis to Identify Proteins Involved in the Uptake and Intracellular Trafficking of Nanoparticles.Small methods · 2026Article
- What is known and unknown about the role of neuroendocrine genesFrontiers in endocrinology · 2025Review
- Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction.Biomolecules · 2022Review
- Isolation of Autophagy Competent Lysosomes from Cancer Cells by Differential Large-Scale Multilayered Density Gradient Centrifugations.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Understanding intracellular nanoparticle trafficking fates through spatiotemporally resolved magnetic nanoparticle recovery.Nanoscale advances · 2021Review
- Isolation and Proteomics of the Insulin Secretory Granule.Metabolites · 2021Review
- Auxin Metabolism in Plants.Cold Spring Harbor perspectives in biology · 2021Review
- A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor.PloS one · 2021Article
- A fluorescent timer reporter enables sorting of insulin secretory granules by age.The Journal of biological chemistry · 2020Article
- Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-Density Gradient.Bio-protocol · 2019Article
- Myosin 1b and F-actin are involved in the control of secretory granule biogenesis.Scientific reports · 2017Article
- The N terminus of pro-endothelial monocyte-activating polypeptide II (EMAP II) regulates its binding with the C terminus, arginyl-tRNA synthetase, and neurofilament light protein.The Journal of biological chemistry · 2015Article
- Genome-wide features of neuroendocrine regulation in Drosophila by the basic helix-loop-helix transcription factor DIMMED.Nucleic acids research · 2015Article
- Article
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
- Subcellular fractionation enhances proteome coverage of pancreatic duct cells.Biochimica et biophysica acta · 2013Article
- Role of adaptor proteins in secretory granule biogenesis and maturation.Frontiers in endocrinology · 2013Article
- Review on recent advances in the analysis of isolated organelles.Analytica chimica acta · 2012Review
- Article
- Determining the number of specific proteins in cellular compartments by quantitative microscopy.Nature protocols · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The field of organellar proteomics has emerged as an attempt to minimize the complexity of the proteomics data obtained from whole cell and tissue extracts while maximizing the resolution on the protein composition of a single subcellular compartment. Standard methods involve lengthy density-based gradient and/or immunoaffinity purification steps followed by extraction, 1-DE or 2-DE, gel staining, in-gel tryptic digestion, and protein identification by MS. In this paper, we present an alternate approach to purify subcellular organelles containing a fluorescent reporter molecule. The gel-free procedure involves fluorescence-assisted sorting of the secretory granules followed by gentle extraction in a buffer compatible with tryptic digestion and MS. Once the subcellular organelle labeled, this procedure can be done in a single day, requires no major modification to any instrumentation and can be readily adapted to the study of other organelles. When applied to corticotrope secretory granules, it led to a much enriched granular fraction from which numerous proteins could be identified through MS.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.