ArticleTransgenic research2009
ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- AnMicroorganisms · 2026Article
- Real-Time PCR Method for Assessment of ParA-Mediated Recombination Efficiency in Minicircle Production.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Split-Cre mediated deletion of DNA no longer needed after site-specific integration in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Efficient Gene Stacking in Rice Using the GAANTRY System.Rice (New York, N.Y.) · 2021Article
- Phenotypic Examination ofPlants (Basel, Switzerland) · 2020Article
- Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane.Plant molecular biology · 2019Article
- Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA.Plant biotechnology journal · 2017Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Review
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.BMC biotechnology · 2012Article
- Improved FLP recombinase, FLPe, efficiently removes marker gene from transgene locus developed by Cre-lox mediated site-specific gene integration in rice.Molecular biotechnology · 2011Article
- Transgene excision in pollen using a codon optimized serine resolvase CinH-RS2 site-specific recombination system.Plant molecular biology · 2011Article
- Novel sulI binary vectors enable an inexpensive foliar selection method in Arabidopsis.BMC research notes · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome.BMC biotechnology · 2010Article
Corrections and comments
- Erratum issuedNunes, Wylla M [added]
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The small serine resolvase ParA from bacterial plasmids RK2 and RP4 catalyzes the recombination of two identical 133 bp recombination sites known as MRS. Previously, we reported that ParA is active in the fission yeast Schizosaccharomyces pombe. In this work, the parA recombinase gene was placed under the control of the Arabidopsis OXS3 promoter and introduced into Arabidopsis lines harboring a chromosomally integrated MRS-flanked target. The ParA recombinase excised the MRS-flanked DNA and the excision event was detected in subsequent generations in the absence of ParA, indicating germinal transmission of the excision event. The precise site-specific deletion by the ParA recombination system in planta demonstrates that the ParA recombinase can be used to remove transgenic DNA, such as selectable markers or other introduced transgenes that are no longer desired in the final product.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.