ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2008
Local DNA underreplication correlates with accumulation of phosphorylated H2Av in the Drosophila melanogaster polytene chromosomes.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Replication timing analysis in polyploid cells reveals Rif1 uses multiple mechanisms to promote underreplication in Drosophila.Genetics · 2021Article
- Effects of Mutations in theCells · 2020Article
- "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.International journal of molecular sciences · 2020Article
- Arp2/3 and Unc45 maintain heterochromatin stability inExperimental biology and medicine (Maywood, N.J.) · 2019Article
- Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster.Chromosoma · 2019Article
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- E2F/DP Prevents Cell-Cycle Progression in Endocycling Fat Body Cells by Suppressing dATM Expression.Developmental cell · 2017Article
- Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile.Epigenetics & chromatin · 2017Article
- Polyteny: still a giant player in chromosome research.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Review
- DNA Replication Control DuringGenetics · 2017Review
- Guanine quadruplex monoclonal antibody 1H6 cross-reacts with restrained thymidine-rich single stranded DNA.Nucleic acids research · 2017Article
- The Drosophila telomere-capping protein Verrocchio binds single-stranded DNA and protects telomeres from DNA damage response.Nucleic acids research · 2017Article
- Replication fork instability and the consequences of fork collisions from rereplication.Genes & development · 2016Review
- Chromosomal Replication Complexity: A Novel DNA Metrics and Genome Instability Factor.PLoS genetics · 2016Review
- Guanine quadruplex structures localize to heterochromatin.Nucleic acids research · 2016Article
- Understanding replication fork progression, stability, and chromosome fragility by exploiting the Suppressor of Underreplication protein.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Heterochromatin formation via recruitment of DNA repair proteins.Molecular biology of the cell · 2015Article
- DNA copy-number control through inhibition of replication fork progression.Cell reports · 2014Article
- Incomplete replication generates somatic DNA alterations within Drosophila polytene salivary gland cells.Genes & development · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA in Drosophila melanogaster polytene chromosomes is known to be locally underreplicated in both pericentric and intercalary heterochromatin. When the SuUR gene is mutant, complete and partial suppression of underreplication are observed in intercalary and pericentric heterochromatin, respectively; in contrast, overexpression of SuUR results in stronger underreplication. Using antibodies against phosphorylated histone H2Av and flies with different levels of SuUR expression, we demonstrated a clear correlation between the extent of underreplication in specific chromosome regions and the accumulation of H2Av phosphorylated at S137 (gamma-H2AX) at the same sites. Phosphorylated H2Av is a well-established marker of DNA double-stranded breaks (DSB). Our data thus argue that DNA underreplication leads to DSBs and that DSBs accumulate as salivary gland cells progress throughout repeated endocycles. We speculate that ligation of free double-stranded DNA termini causes the formation of ectopic contacts between the underreplicated regions in heterochromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.