ArticleThe Journal of clinical investigation2008
Drosophila are protected from Pseudomonas aeruginosa lethality by transgenic expression of paraoxonase-1.
Article in The Journal of clinical investigation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 81 citations in OpenAlex.
- Calprotectin protectsmSystems · 2025Article
- Human Paraoxonase 1: From Bloodstream Enzyme to Disease Fighter & Therapeutic Intervention.Current protein & peptide science · 2025Review
- Targeting microbial quorum sensing: the next frontier to hinder bacterial driven gastrointestinal infections.Gut microbes · 2023Review
- Thermostable Lactonases InhibitInternational journal of molecular sciences · 2023Article
- Review
- Article
- Innovative microbial disease biocontrol strategies mediated by quorum quenching and their multifaceted applications: A review.Frontiers in plant science · 2022Review
- Pseudomonas aeruginosa Quorum Sensing.Advances in experimental medicine and biology · 2022Article
- Article
- Quorum sensing across bacterial and viral domains.PLoS pathogens · 2021Article
- Quorum Sensing Inhibition by Marine Bacteria.Marine drugs · 2019Review
- Bacterial quorum sensing in complex and dynamically changing environments.Nature reviews. Microbiology · 2019Review
- Individual variations in cardiovascular-disease-related protein levels are driven by genetics and gut microbiome.Nature genetics · 2018Article
- Review
- Host modification of a bacterial quorum-sensing signal induces a phenotypic switch in bacterial symbionts.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Paraoxonases-1, -2 and -3: What are their functions?Chemico-biological interactions · 2016Article
- Analysis of esterase enzyme activity in adults of the major malaria vector Anopheles funestus.Parasites & vectors · 2016Article
- Review
- Human pathogenic bacteria, fungi, and viruses in Drosophila: disease modeling, lessons, and shortcomings.Virulence · 2014Review
- Inhaled lactonase reduces Pseudomonas aeruginosa quorum sensing and mortality in rat pneumonia.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa uses quorum sensing, an interbacterial communication system, to regulate gene expression. The signaling molecule N-3-oxododecanoyl homoserine lactone (3OC12-HSL) is thought to play a central role in quorum sensing. Since 3OC12-HSL can be degraded by paraoxonase (PON) family members, we hypothesized that PONs regulate P. aeruginosa virulence in vivo. We chose Drosophila melanogaster as our model organism because it has been shown to be a tractable model for investigating host-pathogen interactions and lacks PONs. By using quorum-sensing-deficient P. aeruginosa, synthetic acyl-HSLs, and transgenic expression of human PON1, we investigated the role of 3OC12-HSL and PON1 on P. aeruginosa virulence. We found that P. aeruginosa virulence in flies was dependent upon 3OC12-HSL. PON1 transgenic flies expressed enzymatically active PON1 and thereby exhibited arylesterase activity and resistance to organophosphate toxicity. Moreover, PON1 flies were protected from P. aeruginosa lethality, and protection was dependent on the lactonase activity of PON1. Our findings show that PON1 can interfere with quorum sensing in vivo and provide insight into what we believe is a novel role for PON1 in the innate immune response to quorum-sensing-dependent pathogens. These results raise intriguing possibilities about human-pathogen interactions, including potential roles for PON1 as a modifier gene and for PON1 protein as a regulator of normal bacterial florae, a link between infection/inflammation and cardiovascular disease, and a potential therapeutic modality.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.