ArticleAmerican journal of respiratory cell and molecular biology2009
Differential gene expression in human conducting airway surface epithelia and submucosal glands.
Article in American journal of respiratory cell and molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets.Respiratory research · 2023Article
- Cell-intrinsic differences between human airway epithelial cells from children and adults.iScience · 2022Article
- Physiology and pathophysiology of human airway mucus.Physiological reviews · 2022Review
- Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.Science advances · 2022Article
- Identification of key genes and pathways in chronic rhinosinusitis with nasal polyps and asthma comorbidity using bioinformatics approaches.Frontiers in immunology · 2022Article
- Network and co-expression analysis of airway smooth muscle cell transcriptome delineates potential gene signatures in asthma.Scientific reports · 2021Article
- Stem Cell-derived Respiratory Epithelial Cell Cultures as Human Disease Models.American journal of respiratory cell and molecular biology · 2021Review
- Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia.American journal of respiratory and critical care medicine · 2021Article
- Article
- The transcriptional signature associated with human motile cilia.Scientific reports · 2020Article
- Dissecting the cellular specificity of smoking effects and reconstructing lineages in the human airway epithelium.Nature communications · 2020Article
- Endotracheal tube mucus as a source of airway mucus for rheological study.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Article
- A Novel Tobacco Use Phenotype Suggests the 15q25 and 19q13 Loci May be Differentially Associated With Cigarettes per Day and Tobacco-Related Problems.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2017Article
- Development of glandular models from human nasal progenitor cells.American journal of respiratory cell and molecular biology · 2015Article
- p40phox expression regulates neutrophil recruitment and function during the resolution phase of intestinal inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Human bronchial epithelial cells differentiate to 3D glandular acini on basement membrane matrix.American journal of respiratory cell and molecular biology · 2011Article
- In vivo function of airway epithelial TLR2 in host defense against bacterial infection.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Glucose depletion in the airway surface liquid is essential for sterility of the airways.PloS one · 2011Article
- Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children.American journal of respiratory and critical care medicine · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Human conducting airways contain two anatomically distinct epithelial cell compartments: surface epithelium and submucosal glands (SMG). Surface epithelial cells interface directly with the environment and function in pathogen detection, fluid and electrolyte transport, and mucus elevation. SMG secrete antimicrobial molecules and most of the airway surface fluid. Despite the unique functional roles of surface epithelia and SMG, little is known about the differences in gene expression and cellular metabolism that orchestrate the specialized functions of these epithelial compartments. To approach this problem, we performed large-scale transcript profiling using epithelial cell samples obtained by laser capture microdissection (LCM) of human bronchus specimens. We found that SMG expressed high levels of many transcripts encoding known or putative innate immune factors, including lactoferrin, zinc alpha-2 glycoprotein, and proline-rich protein 4. By contrast, surface epithelial cells expressed high levels of genes involved in basic nutrient catabolism, xenobiotic clearance, and ciliated structure assembly. Selected confirmation of differentially expressed genes in surface and SMG epithelia demonstrated the predictive power of this approach in identifying genes with localized tissue expression. To characterize metabolic differences between surface epithelial cells and SMG, immunostaining for a mitochondrial marker (isocitrate dehydrogenase) was performed. Because greater staining was observed in the surface compartment, we predict that these cells use significantly more energy than SMG cells. This study illustrates the power of LCM in defining the roles of specific anatomic features in airway biology and may be useful in examining how disease states alter transcriptional programs in the conducting airways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.