ArticleJournal of experimental botany2008
Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content.
Article in Journal of experimental botany, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 195 citations in OpenAlex.
- A hickory diacylglycerol acyltransferase gene coordinates fatty acid metabolism and aluminum tolerance in plants.The New phytologist · 2026Article
- Mechanisms and Advances in Plant Lipid Regulatory Responses Under Biotic and Abiotic Stress.Genes · 2026Review
- Overexpression of Chlorella ellipsoidea DGAT1 gene in soybean improves oil content and influences lipid metabolism.Planta · 2026Article
- Lipid regulation inFrontiers in plant science · 2026Review
- Enhanced seed oil content by overexpressing LPAAT and WRI1 genes in tobacco plant.Plant cell reports · 2025Article
- Article
- A Novel Soybean Diacylglycerol Acyltransferase 1b Variant with Three Amino Acid Substitutions Increases Seed Oil Content.Plant & cell physiology · 2024Article
- The functional divergence of homologous GPAT9 genes contributes to the erucic acid content of Brassica napus seeds.BMC plant biology · 2024Article
- Polyunsaturated Fatty Acids: Conversion to Lipid Mediators, Roles in Inflammatory Diseases and Dietary Sources.International journal of molecular sciences · 2023Review
- Genome-Wide Identification, Classification, and Expression Analyses of theInternational journal of molecular sciences · 2023Article
- Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame.Frontiers in plant science · 2023Article
- BnaPPT1 is essential for chloroplast development and seed oil accumulation in Brassica napus.Journal of advanced research · 2022Article
- Management of plant central metabolism by SnRK1 protein kinases.Journal of experimental botany · 2022Review
- Longer Duration of Active Oil Biosynthesis during Seed Development Is Crucial for High Oil Yield-Lessons from Genome-Wide In Silico Mining and RNA-Seq Validation in Sesame.Plants (Basel, Switzerland) · 2022Article
- Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives.Plant communications · 2022Review
- Genome-Wide Characterization of DGATs and Their Expression Diversity Analysis in Response to Abiotic Stresses inPlants (Basel, Switzerland) · 2022Article
- Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation.The Biochemical journal · 2022Article
- CRISPR-Cas9-mediated editing of starch branching enzymes results in altered starch structure in Brassica napus.Plant physiology · 2022Article
- Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism.Scientific reports · 2022Article
- Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.PloS one · 2022Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Top-down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic pathways that might be overcome by biotechnological approaches. Previous studies on lipid accumulation in oilseed rape have suggested that diacylglycerol acyltransferase (DGAT), which catalyses the final step in seed oil biosynthesis, might be an effective target for enhancing seed oil content. Here, increased seed oil content, increased DGAT activity, and reduced substrate:product ratio are demonstrated, as well as reduced flux control by complex lipid assembly, as determined by TDCA in Brassica napus (canola) lines which overexpress the gene encoding type-1 DGAT. Lines overexpressing DGAT1 also exhibited considerably enhanced seed oil content under drought conditions. These results support the use of TDCA in guiding the rational selection of molecular targets for oilseed modification. The most effective lines had a seed oil increase of 14%. Moreover, overexpression of DGAT1 under drought conditions reduced this environmental penalty on seed oil content.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.