ArticleExperimental neurology2008
Maintaining epitheliopoietic potency when culturing olfactory progenitors.
Article in Experimental neurology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Olfactory models as a platform for neuroscience research.Frontiers in cellular neuroscience · 2026Review
- Strategies to Develop Regenerative Medicine Approaches for Olfactory Disorders.Clinical and experimental otorhinolaryngology · 2025Review
- Organoid models for chemosensing cell studies.American journal of physiology. Cell physiology · 2025Review
- Quiescent horizontal basal stem cells act as a niche for olfactory neurogenesis in a mouse 3D organoid model.Cell reports methods · 2025Article
- An in vitro model of acute horizontal basal cell activation reveals gene regulatory networks underlying the nascent activation phase.Stem cell reports · 2024Article
- Activating a Reserve Neural Stem Cell Population In Vitro Enables Engraftment and Multipotency after Transplantation.Stem cell reports · 2019Article
- CanonicalThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Isolation, culture optimization and functional characterization of stem cell neurospheres from mouse neonatal olfactory bulb and epithelium.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2017Article
- Stem and progenitor cells of the mammalian olfactory epithelium: Taking poietic license.The Journal of comparative neurology · 2017Review
- Contribution of Polycomb group proteins to olfactory basal stem cell self-renewal in a novel c-KIT+ culture model and in vivo.Development (Cambridge, England) · 2016Article
- Pathophysiology of Olfactory Disorders and Potential Treatment Strategies.Current otorhinolaryngology reports · 2016Article
- Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing.Developmental neurobiology · 2016Article
- Translational potential of olfactory mucosa for the study of neuropsychiatric illness.Translational psychiatry · 2015Review
- Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Immunohistological and electrophysiological characterization of Globose basal stem cells.Iranian journal of basic medical sciences · 2014Article
- Molecular events in the cell types of the olfactory epithelium during adult neurogenesis.Molecular brain · 2013Article
- Isolating globose Basal stem cells from albino wistar rats using a highly specific monoclonal antibody.Journal of clinical and diagnostic research : JCDR · 2013Article
- Primary culture of embryonic rat olfactory receptor neurons.In vitro cellular & developmental biology. Animal · 2012Article
- Activin and GDF11 collaborate in feedback control of neuroepithelial stem cell proliferation and fate.Development (Cambridge, England) · 2011Article
- The generation of olfactory epithelial neurospheres in vitro predicts engraftment capacity following transplantation in vivo.Experimental neurology · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The olfactory epithelium is remarkable for the persistence of multipotent, neurocompetent progenitor and stem cells throughout life that can replace all of the various cell types of the epithelium following injury. The therapeutic exploitation of the neurocompetent stem cells of the adult olfactory epithelium would be facilitated by the development of a culture system that maintains the in vivo potency of the progenitors while they are expanded and/or manipulated. We have used an air-liquid interface culture protocol, in which a feeder cell layer of 3T3 cells is established on the underside of a culture insert and Facs-isolated or unsorted progenitor cells from the methyl bromide-lesioned adult rodent epithelium are seeded on upper side. Under these conditions, epithelial cells other than HBCs are capable of organizing themselves into complex three-dimensional, epithelium-lined spheres, which can be passaged. The spheres contain cells with the molecular phenotype of globose basal cells, horizontal basal cells, sustentacular cells and neurons. Spheres derived from mice that express the green fluorescent protein constitutively can be dissociated after 6 days in vitro and directly transplanted into the epithelium of wild-type, methyl bromide-lesioned mice via nasal infusion. The resulting clones contain the various cell types observed in aggregate when globose basal cells are transplanted acutely. In contrast, the same cells cultured as two-dimensional, submerged cultures undergo fibroblastic transition after transplantation and do not integrate into the epithelium. In conclusion, the culture system described here maintains the potency of progenitors, which can then participate in epitheliopoiesis in vivo.
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