ArticleBiochemistry2008
Selective recognition of a DNA G-quadruplex by an engineered antibody.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- RNA G-quadruplex structure targeting and imaging: recent advances and future directions.RNA (New York, N.Y.) · 2025Review
- Structural basis of G-quadruplex recognition by a camelid antibody fragment.Nucleic acids research · 2025Article
- In vivo dynamics and regulation of DNA G-quadruplex structures in mammals.Cell & bioscience · 2023Review
- G-Quadruplex Recognition by DARPIns through Epitope/Paratope Analogy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons.eLife · 2020Article
- Fundamentals of G-quadruplex biology.Annual reports in medicinal chemistry · 2020Article
- G-quadruplex DNA drives genomic instability and represents a targetable molecular abnormality in ATRX-deficient malignant glioma.Nature communications · 2019Article
- Potential Roles for G-Quadruplexes in Mitochondria.Current medicinal chemistry · 2019Review
- The Interplay between G-quadruplex and Transcription.Current medicinal chemistry · 2019Review
- Autoantibody Profiling in Lupus Patients using Synthetic Nucleic Acids.Scientific reports · 2018Article
- Altered biochemical specificity of G-quadruplexes with mutated tetrads.Nucleic acids research · 2016Article
- Structure, Properties, and Biological Relevance of the DNA and RNA G-Quadruplexes: Overview 50 Years after Their Discovery.Biochemistry. Biokhimiia · 2016Article
- Combining Yeast Display and Competitive FACS to Select Rare Hapten-Specific Clones from Recombinant Antibody Libraries.Analytical chemistry · 2016Article
- G-quadruplexes in viruses: function and potential therapeutic applications.Nucleic acids research · 2014Review
- DNA and RNA quadruplex-binding proteins.International journal of molecular sciences · 2014Review
- G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins.Nucleic acids research · 2014Article
- U3 region in the HIV-1 genome adopts a G-quadruplex structure in its RNA and DNA sequence.Biochemistry · 2014Article
- Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells.Nature chemistry · 2014Article
- G-quadruplexes as potential therapeutic targets for embryonal tumors.Molecules (Basel, Switzerland) · 2013Review
- Strand invasion of DNA quadruplexes by PNA: comparison of homologous and complementary hybridization.Chembiochem : a European journal of chemical biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Particular guanine rich nucleic acid sequences can fold into stable secondary structures called G-quadruplexes. These structures have been identified in various regions of the genome that include the telomeres, gene promoters and UTR regions, raising the possibility that they may be associated with biological function(s). Computational analysis has predicted that intramolecular G-quadruplex forming sequences are prevalent in the human genome, thus raising the desire to differentially recognize genomic G-quadruplexes. We have employed antibody phage display and competitive selection techniques to generate a single-chain antibody that shows >1000-fold discrimination between G-quadruplex and duplex DNA, and furthermore >100-fold discrimination between two related intramolecular parallel DNA G-quadruplexes. The amino acid sequence composition at the antigen binding site shows conservation within the light and heavy chains of the selected scFvs, suggesting sequence requirements for G-quadruplex recognition. Circular dichroism (CD) spectroscopic data showed that the scFv binds to the prefolded G-quadruplex and does not induce G-quadruplex structure formation. This study demonstrates the strongest discrimination that we are aware of between two intramolecular genomic G-quadruplexes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.