ArticleBiochemistry2008
Distortion-dependent unhooking of interstrand cross-links in mammalian cell extracts.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Who cites it
31 citing papers in PubMed.
- Transcription-coupled repair of crosslinking DNA damage.DNA repair · 2025Review
- Replication-Independent ICL Repair: From Chemotherapy to Cell Homeostasis.Journal of molecular biology · 2024Review
- Fanconi anemia-independent DNA inter-strand crosslink repair in eukaryotes.Progress in biophysics and molecular biology · 2020Review
- Sensing and Processing of DNA Interstrand Crosslinks by the Mismatch Repair Pathway.Cell reports · 2017Article
- Mechanisms of DNA damage, repair, and mutagenesis.Environmental and molecular mutagenesis · 2017Review
- The role of ADP-ribosylation in regulating DNA interstrand crosslink repair.Journal of cell science · 2016Article
- Involvement of translesion synthesis DNA polymerases in DNA interstrand crosslink repair.DNA repair · 2016Review
- Synthesis of structurally diverse major groove DNA interstrand crosslinks using three different aldehyde precursors.Nucleic acids research · 2014Article
- The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand crosslinks.Molecular cell · 2013Article
- Advances in understanding the complex mechanisms of DNA interstrand cross-link repair.Cold Spring Harbor perspectives in biology · 2013Review
- The differences between ICL repair during and outside of S phase.Trends in biochemical sciences · 2013Review
- The Fanconi anemia pathway in replication stress and DNA crosslink repair.Cellular and molecular life sciences : CMLS · 2012Review
- Repair of cisplatin-induced DNA interstrand crosslinks by a replication-independent pathway involving transcription-coupled repair and translesion synthesis.Nucleic acids research · 2012Article
- Replication-independent repair of DNA interstrand crosslinks.Molecular cell · 2012Article
- Orchestrating the nucleases involved in DNA interstrand cross-link (ICL) repair.Cell cycle (Georgetown, Tex.) · 2011Review
- Probing DNA interstrand cross-link formation by an oxidized abasic site using nonnative nucleotides.Bioorganic & medicinal chemistry · 2011Article
- Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.Genes & development · 2011Article
- DNA interstrand crosslink repair and cancer.Nature reviews. Cancer · 2011Review
- Syntheses of DNA duplexes that contain a N⁴C-alkyl-N⁴C interstrand cross-link.Current protocols in nucleic acid chemistry · 2011Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Interstrand cross-links (ICLs) are formed by many chemotherapeutic agents and may also arise endogenously. The mechanisms used to repair these lesions remain unclear in mammalian cells. Repair in Escherichia coli and Saccharomyces cerevisiae requires an initial unhooking step to release the tethered DNA strands. We used a panel of linear substrates containing different site-specific ICLs to characterize how structure affects ICL processing in mammalian cell extracts. We demonstrate that ICL-induced distortions affect NER-dependent and -independent processing events. The NER-dependent pathway produces dual incisions 5' to the site of the ICL as described previously [Bessho, T., et al. (1997) Mol. Cell. Biol. 17 (12), 6822-6830] but does not release the cross-link. Surprisingly, we also found that the interstrand cross-linked duplexes were unhooked in mammalian cell extracts in a manner independent of the NER pathway. Unhooking occurred identically in extracts prepared from human and rodent cells and is dependent on ATP hydrolysis and metal ions. The structure of the unhooked product was characterized and was found to contain the remnant of the cross-link. Both the NER-mediated dual 5' incisions and unhooking reactions were greatly stimulated by ICL-induced distortions, including increased local flexibility and disruption of base pairs surrounding the site of the ICL. These results suggest that in DNA not undergoing transcription or replication, distortions induced by the presence of an ICL could contribute significantly to initial cross-link recognition and processing.
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