ArticleBiochemistry2008
Mitochondrial NADH fluorescence is enhanced by complex I binding.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 98 citations in OpenAlex.
- Early Postmortem Changes in NADH Fluorescence Lifetime Parameters in Rat Skeletal Muscle: Potential for Postmortem Interval Estimation.International journal of molecular sciences · 2026Article
- Microvascular dysfunction and aberrant network activity drive reduced brain oxygenation in a mouse tauopathy model.Research square · 2025Article
- Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution.BMC biology · 2025Article
- Metabolic changes inmBio · 2024Article
- Rapid, high-resolution, non-destructive assessments of metabolic and morphological homogeneity uniquely identify high-grade cervical precancerous lesions.bioRxiv : the preprint server for biology · 2024Article
- Residual Complex I activity and amphidirectional Complex II operation support glutamate catabolism through mtSLP in anoxia.Scientific reports · 2024Article
- Label-free single-cell live imaging reveals fast metabolic switch in T lymphocytes.Molecular biology of the cell · 2024Article
- Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- Multimodal analyses of vitiligo skin identify tissue characteristics of stable disease.JCI insight · 2022Article
- How many molecules of mitochondrial complex I are in a cell?Analytical biochemistry · 2022Article
- Nicotinamide effects on the metabolism of human fibroblasts and keratinocytes assessed by quantitative, label-free fluorescence imaging.Biomedical optics express · 2021Article
- Energy homeostasis is a conserved process: Evidence from Paracoccus denitrificans' response to acute changes in energy demand.PloS one · 2021Article
- Tissue Imaging and Quantification Relying on Endogenous Contrast.Advances in experimental medicine and biology · 2021Review
- Label-free, High-Resolution Optical Metabolic Imaging of Human Cervical Precancers Reveals Potential for Intraepithelial Neoplasia Diagnosis.Cell reports. Medicine · 2020Article
- Microglial metabolic flexibility supports immune surveillance of the brain parenchyma.Nature communications · 2020Article
- Imaging of macrophage mitochondria dynamicsTheranostics · 2020Article
- Optical changes in THP-1 macrophage metabolism in response to pro- and anti-inflammatory stimuli reported by label-free two-photon imaging.Journal of biomedical optics · 2020Article
- MitoRACE: evaluating mitochondrial function in vivo and in single cells with subcellular resolution using multiphoton NADH autofluorescence.The Journal of physiology · 2019Article
- Cellular Compartmentation and the Redox/Nonredox Functions of NADAntioxidants & redox signaling · 2019Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Mitochondrial NADH fluorescence has been a useful tool in evaluating mitochondrial energetics both in vitro and in vivo. Mitochondrial NADH fluorescence is enhanced several-fold in the matrix through extended fluorescence lifetimes (EFL). However, the actual binding sites responsible for NADH EFL are unknown. We tested the hypothesis that NADH binding to Complex I is a significant source of mitochondrial NADH fluorescence enhancement. To test this hypothesis, the effect of Complex I binding on NADH fluorescence efficiency was evaluated in purified protein, and in native gels of the entire porcine heart mitochondria proteome. To avoid the oxidation of NADH in these preparations, we conducted the binding experiments under anoxic conditions in a specially designed apparatus. Purified intact Complex I enhanced NADH fluorescence in native gels approximately 10-fold. However, no enhancement was detected in denatured individual Complex I subunit proteins. In the Clear and Ghost native gels of the entire mitochondrial proteome, NADH fluorescence enhancement was localized to regions where NADH oxidation occurred in the presence of oxygen. Inhibitor and mass spectroscopy studies revealed that the fluorescence enhancement was specific to Complex I proteins. No fluorescence enhancement was detected for MDH or other dehydrogenases in this assay system, at physiological mole fractions of the matrix proteins. These data suggest that NADH associated with Complex I significantly contributes to the overall mitochondrial NADH fluorescence signal and provides an explanation for the well established close correlation of mitochondrial NADH fluorescence and the metabolic state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.