ArticleCells, tissues, organs2009
The leucine-rich amelogenin peptide alters the amelogenin null enamel phenotype.
Article in Cells, tissues, organs, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4.Journal of dental research · 2023Article
- Tooth Enamel and its Dynamic Protein Matrix.International journal of molecular sciences · 2020Review
- Dose-Dependent Rescue of KO Amelogenin Enamel by TransgenesFrontiers in physiology · 2017Article
- The leucine-rich amelogenin protein (LRAP) is primarily monomeric and unstructured in physiological solution.Journal of structural biology · 2015Article
- The flexible structure of the K24S28 region of Leucine-Rich Amelogenin Protein (LRAP) bound to apatites as a function of surface type, calcium, mutation, and ionic strength.Frontiers in physiology · 2014Article
- Analysis of enamel development using murine model systems: approaches and limitations.Frontiers in physiology · 2014Review
- Neutron reflectometry studies of the adsorbed structure of the amelogenin, LRAP.The journal of physical chemistry. B · 2013Article
- Rescue of the murine amelogenin null phenotype with two amelogenin transgenes.European journal of oral sciences · 2011Article
- The role of amelogenin during enamel-crystallite growth and organization in vivo.European journal of oral sciences · 2011Article
- Leucine-rich amelogenin peptides regulate mineralization in vitro.Journal of dental research · 2011Article
- A solution NMR investigation into the murine amelogenin splice-variant LRAP (Leucine-Rich Amelogenin Protein).Biochimica et biophysica acta · 2010Article
- The leucine rich amelogenin protein (LRAP) adsorbs as monomers or dimers onto surfaces.Journal of structural biology · 2010Article
- Consequences for enamel development and mineralization resulting from loss of function of ameloblastin or enamelin.European journal of oral sciences · 2009Article
- Truncated amelogenin and LRAP transgenes improve Amelx null mouse enamel.Matrix biology : journal of the International Society for Matrix BiologyArticle
- Leucine rich amelogenin peptide alters ameloblast differentiation in vivo.Matrix biology : journal of the International Society for Matrix BiologyArticle
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
introductionThe amelogenin proteins secreted by ameloblasts during dental enamel development are required for normal enamel structure. Amelx null (KO) mice have hypoplastic, disorganized enamel similar to that of human patients with mutations in the AMELX gene, and provide a model system for studies of the enamel defect amelogenesis imperfecta. Because many amelogenin proteins are present in developing enamel due to RNA alternative splicing and proteolytic processing, understanding the function of individual amelogenins has been challenging. PURPOSE: Our objective was to better understand the role of LRAP, a 59 amino acid leucine-rich amelogenin peptide, in the development of enamel. APPROACH: Teeth from transgenic mice that express LRAP under control of the Amelx regulatory regions were analyzed for mechanical properties, and transgenic males were mated with female KO mice. Male offspring with a null background that were transgene positive or transgene negative were compared to determine phenotypic differences using microcomputed tomography (microCT) and scanning electron microscopy (SEM).
resultsNanoindentation revealed no differences between LRAP transgenic and wild-type murine enamel. Using microCT, LRAPKO enamel volume and density measurements were similar to those from KO mice. However, in etched samples examined by SEM, the organization of the enamel rod pattern was altered by the presence of the LRAP transgene.
conclusionsThe presence of LRAP leads to changes in enamel appearance compared to enamel from KO mice. Expression of a combination of amelogenin transgenes in KO mice may lead to rescue of the individual characteristics of normal enamel.
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