ArticleMolecular biology of the cell2008
Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 93 citations in OpenAlex.
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Centromeres drive and take a break.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation.PLoS genetics · 2025Article
- Interactions with multiple inner kinetochore proteins determine mitotic localization of FACT.The Journal of cell biology · 2025Article
- Article
- Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification.Life science alliance · 2025Article
- Concatemer Assisted Stoichiometry Analysis (CASA): targeted mass spectrometry for protein quantification.bioRxiv : the preprint server for biology · 2024Article
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Centromeric chromatin clearings demarcate the site of kinetochore formation.bioRxiv : the preprint server for biology · 2024Article
- Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.Molecular biology of the cell · 2023Article
- Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.Science advances · 2023Article
- Repression of CENP-A assembly in metaphase requires HJURP phosphorylation and inhibition by M18BP1.The Journal of cell biology · 2023Article
- Nanoscale structural organization and stoichiometry of the budding yeast kinetochore.The Journal of cell biology · 2023Article
- Multi-site phosphorylation of yeast Mif2/CENP-C promotes inner kinetochore assembly.Current biology : CB · 2023Article
- Cell cycle control of kinetochore assembly.Nucleus (Austin, Tex.) · 2022Review
- The Four Causes: The Functional Architecture of Centromeres and Kinetochores.Annual review of genetics · 2022Review
- Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.The Journal of cell biology · 2022Article
- Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.Molecular cell · 2022Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Centromere Identity and the Regulation of Chromosome Segregation.Frontiers in cell and developmental biology · 2022Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Mif2p is the budding-yeast orthologue of the mammalian centromere-binding protein CENP-C. We have mapped domains of Saccharomyces cerevisiae Mif2p and studied the phenotyptic consequences of their deletion. Using chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays, we have further shown that Mif2p binds in the CDEIII region of the budding-yeast centromere, probably in close spatial association with Ndc10p. Moreover, ChIP experiments show that Mif2p recruits to yeast kinetochores a substantial subset of inner and outer kinetochore proteins, but not the Ndc80 or Spc105 complexes. We have determined the crystal structure of the C-terminal, dimerization domain of Mif2p. It has a "cupin" fold, extremely similar both in polypeptide chain conformation and in dimer geometry to the dimerization domain of a bacterial transcription factor. The Mif2p dimer seems to be part of an enhanceosome-like structure that nucleates kinetochore assembly in budding yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.