ArticleMolecular biology of the cell2008
A protein methylation pathway in Chlamydomonas flagella is active during flagellar resorption.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Hyperglycemic stress aggravates diabetic retinopathy and nephropathy by promoting cilium disassembly via a deacetylation- and methylation-mediated regulatory mechanism.PLoS biology · 2026Article
- Primary cilium disassembly - from mechanisms to roles in physiology and disease.Journal of cell science · 2026Review
- Activation of the ciliary kinase CDKL5 is mediated by the cyclin-dependent kinase CDK20/LF2 to control flagellar length.PLoS biology · 2025Article
- The methylome of motile cilia.Molecular biology of the cell · 2024Article
- Flexible BProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Methylation of ciliary dynein motors involves the essential cytosolic assembly factor DNAAF3/PF22.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Responses of a Newly Evolved Auxotroph of Chlamydomonas to BPlant physiology · 2020Article
- The Developmental Process of the Growing Motile Ciliary Tip Region.Scientific reports · 2018Article
- Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption.Molecular biology of the cell · 2017Article
- Mechanism of ciliary disassembly.Cellular and molecular life sciences : CMLS · 2016Review
- A NIMA-related kinase, CNK4, regulates ciliary stability and length.Molecular biology of the cell · 2016Article
- Ciliary/Flagellar Protein Ubiquitination.Cells · 2015Review
- Fundamental shift in vitamin B12 eco-physiology of a model alga demonstrated by experimental evolution.The ISME journal · 2015Article
- Recent advances in primary ciliary dyskinesia genetics.Journal of medical genetics · 2015Review
- Unraveling vitamin B12-responsive gene regulation in algae.Plant physiology · 2014Article
- Centrosomal protein CEP104 (Chlamydomonas FAP256) moves to the ciliary tip during ciliary assembly.Journal of cell science · 2013Article
- Primary cilia and kidney injury: current research status and future perspectives.American journal of physiology. Renal physiology · 2013Review
- S-adenosyl homocysteine hydrolase (SAHH) accelerates flagellar regeneration in Dunaliella salina.Current microbiology · 2013Article
- The eukaryotic flagellum makes the day: novel and unforeseen roles uncovered after post-genomics and proteomics data.Current protein & peptide science · 2012Review
- Stages of ciliogenesis and regulation of ciliary length.Differentiation; research in biological diversity · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During intraflagellar transport (IFT), the regulation of motor proteins, the loading and unloading of cargo and the turnover of flagellar proteins all occur at the flagellar tip. To begin an analysis of the protein composition of the flagellar tip, we used difference gel electrophoresis to compare long versus short (i.e., regenerating) flagella. The concentration of tip proteins should be higher relative to that of tubulin (which is constant per unit length of the flagellum) in short compared with long flagella. One protein we have identified is the cobalamin-independent form of methionine synthase (MetE). Antibodies to MetE label flagella in a punctate pattern reminiscent of IFT particle staining, and immunoblot analysis reveals that the amount of MetE in flagella is low in full-length flagella, increased in regenerating flagella, and highest in resorbing flagella. Four methylated proteins have been identified in resorbing flagella, using antibodies specific for asymmetrically dimethylated arginine residues. These proteins are found almost exclusively in the axonemal fraction, and the methylated forms of these proteins are essentially absent in full-length and regenerating flagella. Because most cells resorb cilia/flagella before cell division, these data indicate a link between flagellar protein methylation and progression through the cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.